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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 12 — Dec. 1, 2011
  • pp: 3387–3392

Nomarski serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media

Ali M. Fard, Ata Mahjoubfar, Keisuke Goda, Daniel R. Gossett, Dino Di Carlo, and Bahram Jalali  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 12, pp. 3387-3392 (2011)

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High-speed high-contrast imaging modalities that enable image acquisition of transparent media without the need for chemical staining are essential tools for a broad range of applications; from semiconductor process monitoring to blood screening. Here we introduce a method for contrast-enhanced imaging of unstained transparent objects that is capable of high-throughput imaging. This method combines the Nomarski phase contrast capability with the ultrahigh frame rate and shutter speed of serial time-encoded amplified microscopy. As a proof of concept, we show imaging of a transparent test structure and white blood cells in flow at a shutter speed of 33 ps and a frame rate of 36.1 MHz using a single-pixel photo-detector. This method is expected to be a valuable tool for high-throughput screening of unstained cells.

© 2011 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.3170) Microscopy : Interference microscopy
(320.7160) Ultrafast optics : Ultrafast technology

ToC Category:

Original Manuscript: October 21, 2011
Revised Manuscript: November 18, 2011
Manuscript Accepted: November 22, 2011
Published: November 29, 2011

Ali M. Fard, Ata Mahjoubfar, Keisuke Goda, Daniel R. Gossett, Dino Di Carlo, and Bahram Jalali, "Nomarski serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media," Biomed. Opt. Express 2, 3387-3392 (2011)

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