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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 12 — Dec. 1, 2011
  • pp: 3387–3392

Nomarski serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media

Ali M. Fard, Ata Mahjoubfar, Keisuke Goda, Daniel R. Gossett, Dino Di Carlo, and Bahram Jalali  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 12, pp. 3387-3392 (2011)
http://dx.doi.org/10.1364/BOE.2.003387


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Abstract

High-speed high-contrast imaging modalities that enable image acquisition of transparent media without the need for chemical staining are essential tools for a broad range of applications; from semiconductor process monitoring to blood screening. Here we introduce a method for contrast-enhanced imaging of unstained transparent objects that is capable of high-throughput imaging. This method combines the Nomarski phase contrast capability with the ultrahigh frame rate and shutter speed of serial time-encoded amplified microscopy. As a proof of concept, we show imaging of a transparent test structure and white blood cells in flow at a shutter speed of 33 ps and a frame rate of 36.1 MHz using a single-pixel photo-detector. This method is expected to be a valuable tool for high-throughput screening of unstained cells.

© 2011 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.3170) Microscopy : Interference microscopy
(320.7160) Ultrafast optics : Ultrafast technology

ToC Category:
Microscopy

History
Original Manuscript: October 21, 2011
Revised Manuscript: November 18, 2011
Manuscript Accepted: November 22, 2011
Published: November 29, 2011

Citation
Ali M. Fard, Ata Mahjoubfar, Keisuke Goda, Daniel R. Gossett, Dino Di Carlo, and Bahram Jalali, "Nomarski serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media," Biomed. Opt. Express 2, 3387-3392 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-12-3387


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References

  1. D. Murphy, “Differential interference contrast (DIC) microscopy and modulation contrast microscopy,” in Fundamentals of Light Microscopy and Digital Imaging (Wiley-Liss, New York, 2001).
  2. E. Salmon and P. Tran, “High-resolution video-enhanced differential interference contrast (VEDIC) light microscope,” in Video Microscopy, G. Sluder and D. Wolf, eds. (Academic, New York, 1998).
  3. H. Ishiwata, M. Itoh, and T. Yatagai, “A new method of three-dimensional measurement by differential interference contrast microscope,” Opt. Commun.260(1), 117–126 (2006). [CrossRef]
  4. E. B. Van Munster, E. K. Winter, and J. A. Aten, “Measurement-based evaluation of optical pathlength distributions reconstructed from simulated differential interference contrast images,” J. Microsc.191(2), 170–176 (1998). [CrossRef] [PubMed]
  5. J. Götze, “Application of Nomarski DIC and cathodoluminescence (CL) microscopy to building materials,” Mater. Charact.60(7), 594–602 (2009). [CrossRef]
  6. M. Sokabe and F. Sachs, “The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy,” J. Cell Biol.111(2), 599–606 (1990). [CrossRef] [PubMed]
  7. D. J. Stephens and V. J. Allan, “Light microscopy techniques for live cell imaging,” Science300(5616), 82–86 (2003). [CrossRef] [PubMed]
  8. G. Verheyen, E. Crabbé, H. Joris, and A. Van Steirteghem, “Simple and reliable identification of the human round spermatid by inverted phase-contrast microscopy,” Hum. Reprod.13(6), 1570–1577 (1998). [CrossRef] [PubMed]
  9. C. L. Curl, T. Harris, P. J. Harris, B. E. Allman, C. J. Bellair, A. G. Stewart, and L. M. D. Delbridge, “Quantitative phase microscopy: a new tool for measurement of cell culture growth and confluency in situ,” Pflugers Arch.448(4), 462–468 (2004). [CrossRef] [PubMed]
  10. M. G. Coulthard, A. Nelson, T. Smith, and J. D. Perry, “Point-of-care diagnostic tests for childhood urinary-tract infection: phase-contrast microscopy for bacteria, stick testing, and counting white blood cells,” J. Clin. Pathol.63(9), 823–829 (2010). [CrossRef] [PubMed]
  11. H. Petty, “High speed microscopy in biomedical research,” Opt. Photonics News15, 40–45 (2004).
  12. K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature458(7242), 1145–1149 (2009). [CrossRef] [PubMed]
  13. B. Jalali, P. Soon-Shiong, and K. Goda, “Breaking speed and sensitivity limits,” Optik Photonik5(2), 32–36 (2010). [CrossRef]
  14. S. Chatterjee, “Design considerations and fabrication techniques of Nomarski reflection microscope,” Opt. Eng.42(8), 2202–2213 (2003). [CrossRef]
  15. C. C. Montarou and T. K. Gaylord, “Analysis and design of modified Wollaston prisms,” Appl. Opt.38(31), 6604–6616 (1999). [CrossRef] [PubMed]
  16. K. Goda, D. R. Solli, K. K. Tsia, and B. Jalali, “Theory of amplified dispersive Fourier transformation,” Phys. Rev. A80(4), 043821 (2009). [CrossRef]
  17. D. R. Solli, J. Chou, and B. Jalali, “Amplified wavelength–time transformation for real-time spectroscopy,” Nat. Photonics2(1), 48–51 (2008). [CrossRef]
  18. J. Chou, O. Boyraz, D. R. Solli, and B. Jalali, “Femtosecond real-time single-shot digitizer,” Appl. Phys. Lett.91(16), 161105 (2007). [CrossRef]
  19. K. Goda, A. Motafakker-Fard, and B. Jalali, “Phase-contrast serial time-encoded amplified microscopy,” in CLEO/Europe and EQEC 2009 Conference Digest (Optical Society of America, 2009), paper CH3_4.
  20. D. Di Carlo, “Inertial microfluidics,” Lab Chip9(21), 3038–3046 (2009). [CrossRef] [PubMed]
  21. D. Di Carlo, D. Irimia, R. G. Tompkins, and M. Toner, “Continuous inertial focusing, ordering, and separation of particles in microchannels,” Proc. Natl. Acad. Sci. U.S.A.104(48), 18892–18897 (2007). [CrossRef] [PubMed]
  22. D. L. Lessor, J. S. Hartman, and R. L. Gordon, “Quantitative surface topography determination by Nomarski reflection microscopy,” J. Opt. Soc. Am.69(2), 357–366 (1979). [CrossRef]
  23. K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express18(10), 10016–10028 (2010). [CrossRef] [PubMed]

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