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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 2 — Feb. 1, 2011
  • pp: 218–230

Multiphoton spectral microscopy for imaging and quantification of tissue glycation

Jo-Ya Tseng, Ara A. Ghazaryan, Wen Lo, Yang-Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin-Yuan Tan, and Chen-Yuan Dong  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 2, pp. 218-230 (2011)

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Tissue glycation from diabetes and aging can result in complications such as renal failure, blindness, nerve damage and vascular diseases. In this work, we applied multiphoton microscopy for imaging and characterizing the extent of tissue glycation. The characteristic features of multiphoton autofluorescence (MPAF) and second harmonic generation (SHG) images as well as MPAF spectra of glycated bovine skin, cornea and aorta were acquired. The analysis of MPAF intensity change accompanying the glycation process shows that collagen is more responsive to the formation of autofluorescent advanced glycation endproducts (AGE) than elastic fibers. Changes in spectral features were also used to estimate the rate of glycation in tissues with intrinsic AF. Our study shows that multiphton imaging may be used for the in vitro investigation of the effects of tissue glycation and that this approach may be used for monitoring AGE formation in the clinical setting.

© 2010 OSA

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: August 2, 2010
Revised Manuscript: November 14, 2010
Manuscript Accepted: December 17, 2010
Published: December 24, 2010

Jo-Ya Tseng, Ara A. Ghazaryan, Wen Lo, Yang-Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin-Yuan Tan, and Chen-Yuan Dong, "Multiphoton spectral microscopy for imaging and quantification of tissue glycation," Biomed. Opt. Express 2, 218-230 (2011)

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