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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 2 — Feb. 1, 2011
  • pp: 331–344

Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization

Lisa Miccio, Andrea Finizio, Roberto Puglisi, Donatella Balduzzi, Andrea Galli, and Pietro Ferraro  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 2, pp. 331-344 (2011)

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Differential image contrast (DIC), through the numerical managing and manipulation of complex wavefronts obtained by digital holography (DH), is investigated. We name the approach Dynamical Differential Holographic Image Contrast (DDHIC). DDHIC dispenses from special optics and/or complex setup configurations with moveable components, as usually occurs in classical DIC, that is not well-suited for investigating objects experiencing dynamic evolution during the measurement. In fact, the technique presented here, is useful for floating samples since it allows, from a single recording, to set a posteriori the best conditions for DIC imaging in conjunction with the numerical focusing feature of DH. By DDHIC, the movies can be easily built-up to offering dynamic representation of phase-contrast along all directions, thus improving the visualization. Furthermore, the dynamic representation is useful for making the proper choice of other key parameters of DIC such as the amount of shear and the bias, with the aim to optimize the visualized phase-contrast imaging as favorite representation for bio-scientists. Investigation is performed on various biological samples.

© 2011 OSA

OCIS Codes
(100.2980) Image processing : Image enhancement
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: October 27, 2010
Revised Manuscript: December 10, 2010
Manuscript Accepted: January 10, 2011
Published: January 13, 2011

Lisa Miccio, Andrea Finizio, Roberto Puglisi, Donatella Balduzzi, Andrea Galli, and Pietro Ferraro, "Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization," Biomed. Opt. Express 2, 331-344 (2011)

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