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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 2 — Feb. 1, 2011
  • pp: 396–407

Non-contact spectroscopic determination of large blood volume fractions in turbid media

Rolf H. Bremmer, Stephen C. Kanick, Nick Laan, Arjen Amelink, Ton G. van Leeuwen, and Maurice C. G. Aalders  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 2, pp. 396-407 (2011)
http://dx.doi.org/10.1364/BOE.2.000396


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Abstract

We report on a non-contact method to quantitatively determine blood volume fractions in turbid media by reflectance spectroscopy in the VIS/NIR spectral wavelength range. This method will be used for spectral analysis of tissue with large absorption coefficients and assist in age determination of bruises and bloodstains. First, a phantom set was constructed to determine the effective photon path length as a function of μa and μs′ on phantoms with an albedo range: 0.02-0.99. Based on these measurements, an empirical model of the path length was established for phantoms with an albedo > 0.1. Next, this model was validated on whole blood mimicking phantoms, to determine the blood volume fractions ρ = 0.12-0.84 within the phantoms (r = 0.993; error < 10%). Finally, the model was proved applicable on cotton fabric phantoms.

© 2011 OSA

OCIS Codes
(000.1430) General : Biology and medicine
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(300.1030) Spectroscopy : Absorption

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: November 23, 2010
Revised Manuscript: January 21, 2011
Manuscript Accepted: January 23, 2011
Published: January 24, 2011

Citation
Rolf H. Bremmer, Stephen C. Kanick, Nick Laan, Arjen Amelink, Ton G. van Leeuwen, and Maurice C. G. Aalders, "Non-contact spectroscopic determination of large blood volume fractions in turbid media," Biomed. Opt. Express 2, 396-407 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-2-396


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