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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 3 — Mar. 1, 2011
  • pp: 620–633

Imaging electrical excitation inside the myocardial wall

Bogdan G Mitrea, Bryan J Caldwell, and Arkady M Pertsov  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 3, pp. 620-633 (2011)

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Cardiac arrhythmias are often triggered by ectopic membrane depolarization originating deep inside the myocardial wall. Here we propose a new method utilizing a novel near-infrared voltage-sensitive fluorescent dye DI-4-ANBDQBS to determine the three-dimensional (3D) coordinates of the sources of such depolarization. We tested the method in live preparations of pig left and right ventricular myocardium (thickness 8-18 mm) and phantoms imitating the optical properties of myocardial tissue. The method utilizes an alternating transillumination approach that involves comparing pairs of simultaneously recorded broad-field epifluorescence and transillumination images produced at two alternating directions of illumination. Recordings were taken simultaneously by two CCD cameras facing the endocardial and epicardial surfaces of the heart at a frame rate up to 3 KHz. In live preparations, we were able to localize the origin of the depolarization wave with a precision of ±1.3mm in the transmural direction and 3 mm in the image plane. The accuracy of detection was independent of the depth of the source inside ventricular wall.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Optics in Cancer Research

Original Manuscript: January 4, 2011
Revised Manuscript: February 11, 2011
Manuscript Accepted: February 13, 2011
Published: February 16, 2011

Bogdan G Mitrea, Bryan J Caldwell, and Arkady M Pertsov, "Imaging electrical excitation inside the myocardial wall," Biomed. Opt. Express 2, 620-633 (2011)

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