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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 739–747

Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms

Rodrigo Aviles-Espinosa, George Filippidis, Craig Hamilton, Graeme Malcolm, Kurt J. Weingarten, Thomas Südmeyer, Yohan Barbarin, Ursula Keller, Susana I.C.O Santos, David Artigas, and Pablo Loza-Alvarez  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 4, pp. 739-747 (2011)

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We present a portable ultrafast Semiconductor Disk Laser (SDL) (or vertical extended cavity surface emitting laser—VECSELs), to be used for nonlinear microscopy. The SDL is modelocked using a quantum-dot semiconductor saturable absorber mirror (SESAM), delivering an average output power of 287 mW, with 1.5 ps pulses at 500 MHz and a central wavelength of 965 nm. Specifically, despite the fact of having long pulses and high repetition rates, we demonstrate the potential of this laser for Two-Photon Excited Fluorescence (TPEF) imaging of in vivo Caenorhabditis elegans (C. elegans) expressing Green Fluorescent Protein (GFP) in a set of neuronal processes and cell bodies. Efficient TPEF imaging is achieved due to the fact that this wavelength matches the peak of the two-photon action cross section of this widely used fluorescent marker. The SDL extended versatility is shown by presenting Second Harmonic Generation images of pharynx, uterus, body wall muscles and its potential to be used to excite other different commercial dyes. Importantly this non-expensive, turn-key, compact laser system could be used as a platform to develop portable nonlinear bio-imaging devices.

© 2011 OSA

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(140.3538) Lasers and laser optics : Lasers, pulsed
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: December 15, 2010
Revised Manuscript: January 14, 2011
Manuscript Accepted: February 24, 2011
Published: February 28, 2011

Rodrigo Aviles-Espinosa, George Filippidis, Craig Hamilton, Graeme Malcolm, Kurt J. Weingarten, Thomas Südmeyer, Yohan Barbarin, Ursula Keller, Susana I.C.O Santos, David Artigas, and Pablo Loza-Alvarez, "Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms," Biomed. Opt. Express 2, 739-747 (2011)

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