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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 748–763

Optics InfoBase > Biomedical Optics Express > Volume 2 > Issue 4 > Page 748

Imaging cone photoreceptors in three dimensions and in time using ultrahigh resolution optical coherence tomography with adaptive optics

Omer P. Kocaoglu, Sangyeol Lee, Ravi S. Jonnal, Qiang Wang, Ashley E. Herde, Jack C. Derby, Weihua Gao, and Donald T. Miller  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 4, pp. 748-763 (2011)
http://dx.doi.org/10.1364/BOE.2.000748


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Abstract

Cone photoreceptors in the living human eye have recently been imaged with micron-scale resolution in all three spatial dimensions using adaptive optics optical coherence tomography. While these advances have allowed non-invasive study of the three-dimensional structure of living human cones, studies of their function and physiology are still hampered by the difficulties to monitor the same cells over time. The purpose of this study is to demonstrate the feasibility of cone monitoring using ultrahigh-resolution adaptive optics optical coherence tomography. Critical to this is incorporation of a high speed CMOS camera (125 KHz) and a novel feature-based, image registration/dewarping algorithm for reducing the deleterious effects of eye motion on volume images. Volume movies were acquired on three healthy subjects at retinal eccentricities from 0.5° to 6°. Image registration/dewarping reduced motion artifacts in the movies from 15 μm to 1.3 μm root mean square, the latter sufficient for identifying and tracking cones. Cone row-to-row spacing and outer segment lengths were consistent with that reported in the literature. Cone length analysis demonstrates that UHR-AO-OCT is sufficiently sensitive to measure real length differences between cones in the same 0.5° retinal patch, and requires no more than five measurements of OS length to achieve 95% confidence. We know of no other imaging modality that can monitor foveal or parafoveal cones over time with comparable resolution in all three dimensions.

© 2011 OSA

OCIS Codes
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: January 4, 2011
Revised Manuscript: February 15, 2011
Manuscript Accepted: February 16, 2011
Published: March 1, 2011

Virtual Issues
Cellular Imaging of the Retina (2011) Biomedical Optics Express

Citation
Omer P. Kocaoglu, Sangyeol Lee, Ravi S. Jonnal, Qiang Wang, Ashley E. Herde, Jack C. Derby, Weihua Gao, and Donald T. Miller, "Imaging cone photoreceptors in three dimensions and in time using ultrahigh resolution optical coherence tomography with adaptive optics," Biomed. Opt. Express 2, 748-763 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-4-748


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