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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 871–886

Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates

Jin Chen, Vivek Venugopal, and Xavier Intes  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 4, pp. 871-886 (2011)

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Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full fluorescence time measurements, a light propagation model that accurately simulates weakly diffused and multiple scattered photons is required. In this article, we derive a computationally efficient Monte Carlo based method to compute time-gated fluorescence Jacobians for the simultaneous imaging of two fluorophores with lifetime contrast. The Monte Carlo based formulation is validated on a synthetic murine model simulating the uptake in the kidneys of two distinct fluorophores with lifetime contrast. Experimentally, the method is validated using capillaries filled with 2.5nmol of ICG and IRDye™800CW respectively embedded in a diffuse media mimicking the average optical properties of mice. Combining multiple time gates in one inverse problem allows the simultaneous reconstruction of multiple fluorophores with increased resolution and minimal crosstalk using the proposed formulation.

© 2011 OSA

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(110.1758) Imaging systems : Computational imaging

ToC Category:
Image Reconstruction and Inverse Problems

Original Manuscript: January 4, 2011
Revised Manuscript: February 25, 2011
Manuscript Accepted: February 26, 2011
Published: March 14, 2011

Jin Chen, Vivek Venugopal, and Xavier Intes, "Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates," Biomed. Opt. Express 2, 871-886 (2011)

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