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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 901–914

Optics InfoBase > Biomedical Optics Express > Volume 2 > Issue 4 > Page 901

Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy

Te-Yu Tseng, Chun-Yu Chen, Yi-Shan Li, and Kung-Bin Sung  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 4, pp. 901-914 (2011)
http://dx.doi.org/10.1364/BOE.2.000901


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Abstract

We applied hyperspectral imaging to measure spatially-resolved diffuse reflectance spectra in the visible range and an iterative inversion method based on forward Monte Carlo modeling to quantify optical properties of two-layered tissue models. We validated the inversion method using spectra experimentally measured from liquid tissue mimicking phantoms with known optical properties. Results of fitting simulated data showed that simultaneously considering the spatial and spectral information in the inversion process improves the accuracies of estimating the optical properties and the top layer thickness in comparison to methods fitting reflectance spectra measured with a single source-detector separation or fitting spatially-resolved reflectance at a single wavelength. Further development of the method could improve noninvasive assessment of physiological status and pathological conditions of stratified squamous epithelium and superficial stroma.

© 2011 OSA

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media
(300.6550) Spectroscopy : Spectroscopy, visible
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Optics of Tissue and Turbid Media

History
Original Manuscript: January 25, 2011
Revised Manuscript: February 17, 2011
Manuscript Accepted: March 6, 2011
Published: March 16, 2011

Citation
Te-Yu Tseng, Chun-Yu Chen, Yi-Shan Li, and Kung-Bin Sung, "Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy," Biomed. Opt. Express 2, 901-914 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-4-901


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References

  1. V. T. C. Chang, P. S. Cartwright, S. M. Bean, G. M. Palmer, R. C. Bentley, and N. Ramanujam, “Quantitative physiology of the precancerous cervix in vivo through optical spectroscopy,” Neoplasia 11(4), 325–332 (2009). [PubMed]
  2. J. Q. Brown, K. Vishwanath, G. M. Palmer, and N. Ramanujam, “Advances in quantitative UV-visible spectroscopy for clinical and pre-clinical application in cancer,” Curr. Opin. Biotechnol. 20(1), 119–131 (2009). [CrossRef] [PubMed]
  3. S. McGee, J. Mirkovic, V. Mardirossian, A. Elackattu, C. C. Yu, S. Kabani, G. Gallagher, R. Pistey, L. Galindo, K. Badizadegan, Z. Wang, R. Dasari, M. S. Feld, and G. Grillone, “Model-based spectroscopic analysis of the oral cavity: impact of anatomy,” J. Biomed. Opt. 13(6), 064034 (2008). [CrossRef] [PubMed]
  4. A. Amelink, O. P. Kaspers, H. J. Sterenborg, J. E. van der Wal, J. L. Roodenburg, and M. J. Witjes, “Non-invasive measurement of the morphology and physiology of oral mucosa by use of optical spectroscopy,” Oral Oncol. 44(1), 65–71 (2008). [CrossRef] [PubMed]
  5. P. R. Bargo, S. A. Prahl, T. T. Goodell, R. A. Sleven, G. Koval, G. Blair, and S. L. Jacques, “In vivo determination of optical properties of normal and tumor tissue with white light reflectance and an empirical light transport model during endoscopy,” J. Biomed. Opt. 10(3), 034018 (2005). [CrossRef] [PubMed]
  6. D. Arifler, C. MacAulay, M. Follen, and R. Richards-Kortum, “Spatially resolved reflectance spectroscopy for diagnosis of cervical precancer: Monte Carlo modeling and comparison to clinical measurements,” J. Biomed. Opt. 11(6), 064027 (2006). [CrossRef] [PubMed]
  7. I. Georgakoudi, B. C. Jacobson, M. G. Müller, E. E. Sheets, K. Badizadegan, D. L. Carr-Locke, C. P. Crum, C. W. Boone, R. R. Dasari, J. Van Dam, and M. S. Feld, “NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes,” Cancer Res. 62(3), 682–687 (2002). [PubMed]
  8. A. Kim, M. Roy, F. Dadani, and B. C. Wilson, “A fiberoptic reflectance probe with multiple source-collector separations to increase the dynamic range of derived tissue optical absorption and scattering coefficients,” Opt. Express 18(6), 5580–5594 (2010). [CrossRef] [PubMed]
  9. D. J. Cuccia, F. Bevilacqua, A. J. Durkin, F. R. Ayers, and B. J. Tromberg, “Quantitation and mapping of tissue optical properties using modulated imaging,” J. Biomed. Opt. 14(2), 024012 (2009). [CrossRef] [PubMed]
  10. R. Zhang, W. Verkruysse, B. Choi, J. A. Viator, B. Jung, L. O. Svaasand, G. Aguilar, and J. S. Nelson, “Determination of human skin optical properties from spectrophotometric measurements based on optimization by genetic algorithms,” J. Biomed. Opt. 10(2), 024030 (2005). [CrossRef] [PubMed]
  11. J. C. Finlay and T. H. Foster, “Hemoglobin oxygen saturations in phantoms and in vivo from measurements of steady-state diffuse reflectance at a single, short source-detector separation,” Med. Phys. 31(7), 1949–1959 (2004). [CrossRef] [PubMed]
  12. G. Zonios, L. T. Perelman, V. M. Backman, R. Manoharan, M. Fitzmaurice, J. Van Dam, and M. S. Feld, “Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo,” Appl. Opt. 38(31), 6628–6637 (1999). [CrossRef] [PubMed]
  13. R. M. P. Doornbos, R. Lang, M. C. Aalders, F. W. Cross, and H. J. Sterenborg, “The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopy,” Phys. Med. Biol. 44(4), 967–981 (1999). [CrossRef] [PubMed]
  14. M. G. Nichols, E. L. Hull, and T. H. Foster, “Design and testing of a white-light, steady-state diffuse reflectance spectrometer for determination of optical properties of highly scattering systems,” Appl. Opt. 36(1), 93–104 (1997). [CrossRef] [PubMed]
  15. Q. Z. Wang, H. Z. Yang, A. Agrawal, N. S. Wang, and T. J. Pfefer, “Measurement of internal tissue optical properties at ultraviolet and visible wavelengths: Development and implementation of a fiberoptic-based system,” Opt. Express 16(12), 8685–8703 (2008). [CrossRef] [PubMed]
  16. R. Reif, O. A’Amar, and I. J. Bigio, “Analytical model of light reflectance for extraction of the optical properties in small volumes of turbid media,” Appl. Opt. 46(29), 7317–7328 (2007). [CrossRef] [PubMed]
  17. G. M. Palmer and N. Ramanujam, “Monte Carlo-based inverse model for calculating tissue optical properties. Part I: Theory and validation on synthetic phantoms,” Appl. Opt. 45(5), 1062–1071 (2006). [CrossRef] [PubMed]
  18. P. Thueler, I. Charvet, F. Bevilacqua, M. St. Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, “In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties,” J. Biomed. Opt. 8(3), 495–503 (2003). [CrossRef] [PubMed]
  19. T. H. Pham, F. Bevilacqua, T. Spott, J. S. Dam, B. J. Tromberg, and S. Andersson-Engels, “Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging,” Appl. Opt. 39(34), 6487–6497 (2000). [CrossRef] [PubMed]
  20. A. Kienle, L. Lilge, M. S. Patterson, R. Hibst, R. Steiner, and B. C. Wilson, “Spatially resolved absolute diffuse reflectance measurements for noninvasive determination of the optical scattering and absorption coefficients of biological tissue,” Appl. Opt. 35(13), 2304–2314 (1996). [CrossRef] [PubMed]
  21. J. S. Dam, C. B. Pedersen, T. Dalgaard, P. E. Fabricius, P. Aruna, and S. Andersson-Engels, “Fiber-optic probe for noninvasive real-time determination of tissue optical properties at multiple wavelengths,” Appl. Opt. 40(7), 1155–1164 (2001). [CrossRef] [PubMed]
  22. M. S. Patterson, B. Chance, and B. C. Wilson, “Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties,” Appl. Opt. 28(12), 2331–2336 (1989). [CrossRef] [PubMed]
  23. B. J. Tromberg, L. O. Svaasand, T. T. Tsay, and R. C. Haskell, “Properties of photon density waves in multiple-scattering media,” Appl. Opt. 32(4), 607–616 (1993). [CrossRef] [PubMed]
  24. A. M. J. Wang, J. E. Bender, J. Pfefer, U. Utzinger, and R. A. Drezek, “Depth-sensitive reflectance measurements using obliquely oriented fiber probes,” J. Biomed. Opt. 10(4), 044017 (2005). [CrossRef] [PubMed]
  25. J. R. Weber, D. J. Cuccia, A. J. Durkin, and B. J. Tromberg, “Noncontact imaging of absorption and scattering in layered tissue using spatially modulated structured light,” J. Appl. Phys. 105(10), 102028 (2009). [CrossRef]
  26. D. Yudovsky and L. Pilon, “Rapid and accurate estimation of blood saturation, melanin content, and epidermis thickness from spectral diffuse reflectance,” Appl. Opt. 49(10), 1707–1719 (2010). [CrossRef] [PubMed]
  27. H. Y. Cen and R. F. Lu, “Quantification of the optical properties of two-layer turbid materials using a hyperspectral imaging-based spatially-resolved technique,” Appl. Opt. 48(29), 5612–5623 (2009). [CrossRef] [PubMed]
  28. A. Kienle, M. S. Patterson, N. Dögnitz, R. Bays, G. Wagnières, and H. van den Bergh, “Noninvasive determination of the optical properties of two-layered turbid media,” Appl. Opt. 37(4), 779–791 (1998). [CrossRef] [PubMed]
  29. T.-Y. Tseng, P.-J. Lai, and K.-B. Sung, “High-throughput detection of immobilized plasmonic nanoparticles by a hyperspectral imaging system based on Fourier transform spectrometry,” Opt. Express 19(2), 1291–1300 (2011). [CrossRef] [PubMed]
  30. Q. Liu and N. Ramanujam, “Scaling method for fast Monte Carlo simulation of diffuse reflectance spectra from multilayered turbid media,” J. Opt. Soc. Am. A 24(4), 1011–1025 (2007). [CrossRef] [PubMed]
  31. L. Wang, S. L. Jacques, and L. Zheng, “MCML--Monte Carlo modeling of light transport in multi-layered tissues,” Comput. Methods Programs Biomed. 47(2), 131–146 (1995). [CrossRef] [PubMed]
  32. R. Graaff, M. H. Koelink, F. F. M. de Mul, W. G. Zijistra, A. C. M. Dassel, and J. G. Aarnoudse, “Condensed Monte Carlo simulations for the description of light transport,” Appl. Opt. 32(4), 426–434 (1993). [CrossRef] [PubMed]
  33. S. Prahl, “Tabulated Molar Extinction Coefficient for Hemoglobin in Water,” http://omlc.ogi.edu/spectra/hemoglobin/summary.html .
  34. I. Pavlova, C. R. Weber, R. A. Schwarz, M. Williams, A. El-Naggar, A. Gillenwater, and R. Richards-Kortum, “Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer,” J. Biomed. Opt. 13(6), 064012 (2008). [CrossRef] [PubMed]
  35. S. Gioux, A. Mazhar, D. J. Cuccia, A. J. Durkin, B. J. Tromberg, and J. V. Frangioni, “Three-dimensional surface profile intensity correction for spatially modulated imaging,” J. Biomed. Opt. 14(3), 034045 (2009). [CrossRef] [PubMed]
  36. D. Arifler, “Sensitivity of spatially resolved reflectance signals to coincident variations in tissue optical properties,” Appl. Opt. 49(22), 4310–4320 (2010). [CrossRef] [PubMed]

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