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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 915–926

Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy

Liang Gao, Haijun Zhou, Michael J. Thrall, Fuhai Li, Yaliang Yang, Zhiyong Wang, Pengfei Luo, Kelvin K. Wong, Ganesh S. Palapattu, and Stephen T. C. Wong  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 4, pp. 915-926 (2011)

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A custom built coherent anti-Stokes Raman scattering (CARS) microscope was used to image prostatic glands and nerve structures from 17 patients undergoing radical prostatectomy. Imaging of glandular and nerve structures showed distinctive cellular features that correlated to histological stains. Segmentation of cell nucleus was performed to establish a cell feature-based model to separate normal glands from cancer glands. In this study, we use a single parameter, average cell neighbor distance based on CARS imaging, to characterize normal and cancerous glandular structures. By combining CARS with our novel classification model, we are able to characterize prostate glandular and nerve structures in a manner that potentially enables real-time, intra-operative assessment of surgical margins and neurovascular bundles. As such, this method could potentially improve outcomes following radical prostatectomy.

© 2011 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Optics in Cancer Research

Original Manuscript: December 20, 2010
Revised Manuscript: February 25, 2011
Manuscript Accepted: March 7, 2011
Published: March 18, 2011

Liang Gao, Haijun Zhou, Michael J. Thrall, Fuhai Li, Yaliang Yang, Zhiyong Wang, Pengfei Luo, Kelvin K. Wong, Ganesh S. Palapattu, and Stephen T. C. Wong, "Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy," Biomed. Opt. Express 2, 915-926 (2011)

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