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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 927–936

Deep UV resonant Raman spectroscopy for photodamage characterization in cells

Yasuaki Kumamoto, Atsushi Taguchi, Nicholas Isaac Smith, and Satoshi Kawata  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 4, pp. 927-936 (2011)

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We employed deep UV (DUV) Raman spectroscopy for characterization of molecular photodamage in cells. 244 nm light excitation Raman spectra were measured for HeLa cells exposed to the excitation light for different durations. In the spectra obtained with the shortest exposure duration (0.25 sec at 16 µW/µm2 irradiation), characteristic resonant Raman bands of adenine and guanine at 1483 cm−1 and tryptophan and tyrosine at 1618 cm−1 were clearly visible. With increasing exposure duration (up to 12.5 sec), these biomolecular Raman bands diminished, while a photoproduct Raman band at 1611 cm−1 grew. By exponential function fitting analyses, intensities of these characteristic three bands were correlated with sample exposure duration at different intensities of excitation light. We then suggest practical excitation conditions effective for DUV Raman observation of cells without photodamage-related spectral distortion.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6450) Spectroscopy : Spectroscopy, Raman
(300.6540) Spectroscopy : Spectroscopy, ultraviolet
(350.1820) Other areas of optics : Damage

ToC Category:
Cell Studies

Original Manuscript: November 12, 2011
Revised Manuscript: February 18, 2011
Manuscript Accepted: March 9, 2011
Published: March 18, 2011

Yasuaki Kumamoto, Atsushi Taguchi, Nicholas Isaac Smith, and Satoshi Kawata, "Deep UV resonant Raman spectroscopy for photodamage characterization in cells," Biomed. Opt. Express 2, 927-936 (2011)

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