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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 987–995

Single-shot slightly-off-axis interferometry based Hilbert phase microscopy of red blood cells

Liang Xue, Jiancheng Lai, Shouyu Wang, and Zhenhua Li  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 4, pp. 987-995 (2011)
http://dx.doi.org/10.1364/BOE.2.000987


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Abstract

A slightly-off-axis interferometry based Hilbert phase microscopy (HPM) method is developed to quantitatively obtain the phase distribution. Owing to its single-shot nature and details detection ability, HPM can be used to investigate rapid phenomena that take place in transparent structures such as biological cells. Moreover, the slightly-off-axis interferometry owns higher effective bandwidth and more sensitivity than traditional off-axis interferometry. The proposed method takes advantages of the above techniques to obtain the phase image of the red blood cells and compared with the traditional off-axis interferometry and phase retrieval algorithm based on the FFT. The experimental results show that the proposed method owns fine spatial details and real-time imaging ability. We are sure that the proposed method provides a breakthrough for real-time observing and quantitative analyzing of cells in vivo.

© 2011 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 18, 2011
Revised Manuscript: March 1, 2011
Manuscript Accepted: March 22, 2011
Published: March 29, 2011

Citation
Liang Xue, Jiancheng Lai, Shouyu Wang, and Zhenhua Li, "Single-shot slightly-off-axis interferometry based Hilbert phase microscopy of red blood cells," Biomed. Opt. Express 2, 987-995 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-4-987


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