OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1194–1203

Optics InfoBase > Biomedical Optics Express > Volume 2 > Issue 5 > Page 1194

Gold nanorods for intravital vascular imaging of preneoplastic oral mucosa

Saam Motamedi, Tuya Shilagard, Kert Edward, Luke Koong, Suimin Qui, and Gracie Vargas  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1194-1203 (2011)
http://dx.doi.org/10.1364/BOE.2.001194


View Full Text Article

Enhanced HTML    Acrobat PDF (826 KB)


Advanced Search

Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We explore the feasibility of using gold nanorods with efficient two-photon luminescence properties as contrast agents for intravital imaging of neoplasia. This investigation spanned ex vivo characterization in cells/tissue to in vivo implementation in an oral carcinogenesis model. GNRs were >40 times brighter than surrounding tissue. Intravital imaging revealed 3D microvasculature, and in dysplasia, abnormal vessels (dense and tortuous) compared to normal. GNRs were diffusely distributed in lesions after 24 hours. No known previous study has revealed abnormal vessel structure in dysplasia by imaging. Results suggest GNRs can function as high-contrast agents for in vivo visualization of carcinogenesis features.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(160.4236) Materials : Nanomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Nanotechnology and Plasmonics

History
Original Manuscript: January 4, 2011
Revised Manuscript: April 8, 2011
Manuscript Accepted: April 11, 2011
Published: April 13, 2011

Citation
Saam Motamedi, Tuya Shilagard, Kert Edward, Luke Koong, Suimin Qui, and Gracie Vargas, "Gold nanorods for intravital vascular imaging of preneoplastic oral mucosa," Biomed. Opt. Express 2, 1194-1203 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1194


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. X. Huang, I. H. El-Sayed, and M. A. El-Sayed, “Applications of gold nanorods for cancer imaging and photothermal therapy,” Methods Mol. Biol. 624, 343–357 (2010). [CrossRef] [PubMed]
  2. J. Park, A. Estrada, K. Sharp, K. Sang, J. A. Schwartz, D. K. Smith, C. Coleman, J. D. Payne, B. A. Korgel, A. K. Dunn, and J. W. Tunnell, “Two-photon-induced photoluminescence imaging of tumors using near-infrared excited gold nanoshells,” Opt. Express 16(3), 1590–1599 (2008). [CrossRef] [PubMed]
  3. H. Wang, T. B. Huff, D. A. Zweifel, W. He, P. S. Low, A. Wei, and J. X. Cheng, “In vitro and in vivo two-photon luminescence imaging of single gold nanorods,” Proc. Natl. Acad. Sci. U.S.A. 102(44), 15752–15756 (2005). [CrossRef] [PubMed]
  4. N. J. Durr, T. Larson, D. K. Smith, B. A. Korgel, K. Sokolov, and A. Ben-Yakar, “Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods,” Nano Lett. 7(4), 941–945 (2007). [CrossRef] [PubMed]
  5. I. H. El-Sayed, X. H. Huang, and M. A. El-Sayed, “Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles,” Cancer Lett. 239(1), 129–135 (2006). [CrossRef] [PubMed]
  6. M. Eghtedari, A. V. Liopo, J. A. Copland, A. A. Oraevsky, and M. Motamedi, “Engineering of hetero-functional gold nanorods for the in vivo molecular targeting of breast cancer cells,” Nano Lett. 9(1), 287–291 (2009). [CrossRef] [PubMed]
  7. S. E. Gratton, P. A. Ropp, P. D. Pohlhaus, J. C. Luft, V. J. Madden, M. E. Napier, and J. M. DeSimone, “The effect of particle design on cellular internalization pathways,” Proc. Natl. Acad. Sci. U.S.A. 105(33), 11613–11618 (2008). [CrossRef] [PubMed]
  8. X. Qu, J. Wang, Z. Zhang, N. Koop, R. Rahmanzadeh, and G. Hüttmann, “Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes,” J. Biomed. Opt. 13(3), 031217 (2008). [CrossRef] [PubMed]
  9. L. Tong, W. He, Y. Zhang, W. Zheng, and J. X. Cheng, “Visualizing systemic clearance and cellular level biodistribution of gold nanorods by intrinsic two-photon luminescence,” Langmuir 25(21), 12454–12459 (2009). [CrossRef] [PubMed]
  10. American Cancer Society, Cancer Facts & Figures 2002 (American Cancer Society, New York, 2002).
  11. S. Silverman, Oral Cancer, 3rd ed. (The American Cancer Society, Atlanta, 1990).
  12. S. A. Liu, Y. K. Wong, J. C. Lin, C. K. Poon, K. C. Tung, and W. C. Tsai, “Impact of recurrence interval on survival of oral cavity squamous cell carcinoma patients after local relapse,” Otolaryngol. Head Neck Surg. 136(1), 112–118 (2007). [CrossRef] [PubMed]
  13. R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature 452(7187), 580–589 (2008). [CrossRef] [PubMed]
  14. J. Sun, T. Shilagard, B. Bell, M. Motamedi, and G. Vargas, “In vivo multimodal nonlinear optical imaging of mucosal tissue,” Opt. Express 12(11), 2478–2486 (2004). [CrossRef] [PubMed]
  15. J. Paoli, M. Smedh, and M. B. Ericson, “Multiphoton laser scanning microscopy--a novel diagnostic method for superficial skin cancers,” Semin. Cutan. Med. Surg. 28(3), 190–195 (2009). [CrossRef] [PubMed]
  16. P. Wilder-Smith, K. Osann, N. Hanna, N. El Abbadi, M. Brenner, D. Messadi, and T. Krasieva, “In vivo multiphoton fluorescence imaging: a novel approach to oral malignancy,” Lasers Surg. Med. 35(2), 96–103 (2004). [CrossRef] [PubMed]
  17. M. C. Skala, J. M. Squirrell, K. M. Vrotsos, J. C. Eickhoff, A. Gendron-Fitzpatrick, K. W. Eliceiri, and N. Ramanujam, “Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues,” Cancer Res. 65(4), 1180–1186 (2005). [CrossRef] [PubMed]
  18. G. Vargas, T. Shilagard, J. Sun, and M. Motamedi, “In vivo multiphoton and second harmonic generation microscopy of epithelial carcinogenesis,” Proc. SPIE 6091, 000–000 (2006).
  19. M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
  20. G. Vargas, T. Shilagard, K. H. Ho, and S. McCammon, “Multiphoton autofluorescence microscopy and second harmonic generation microscopy of oral epithelial neoplasms,” Conf. Proc. IEEE Eng. Med. Biol. Soc. 2009, 6311–6313 (2009). [PubMed]
  21. V. A. Resto, M. M. Burdick, N. M. Dagia, S. D. McCammon, S. M. Fennewald, and R. Sackstein, “L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditions,” J. Biol. Chem. 283(23), 15816–15824 (2008). [CrossRef] [PubMed]
  22. G. Shklar, “Experimental pathology of oral cancer,” in Oral Cancer, G. Shklar, ed. (W.B. Sanders, 1984), pp. 41–54.
  23. A. Ebihara, T. B. Krasieva, L.-H. Liaw, S. Fago, D. Messadi, K. Osann, and P. Wilder-Smith, “Detection and diagnosis of oral cancer by light-induced fluorescence,” Lasers Surg. Med. 32(1), 17–24 (2003). [CrossRef] [PubMed]
  24. A. M. Kluftinger, N. L. Davis, N. F. Quenville, S. Lam, J. Hung, and B. Palcic, “Detection of squamous cell cancer and pre-cancerous lesions by imaging of tissue autofluorescence in the hamster cheek pouch model,” Surg. Oncol. 1(2), 183–188 (1992). [CrossRef] [PubMed]
  25. V. L. Noonan and S. Kabani, “Diagnosis and management of suspicious lesions of the oral cavity,” Otolaryngol. Clin. North Am. 38(1), 21–35, vii (2005) (vii.). [CrossRef] [PubMed]
  26. F. Nussenbaum and I. M. Herman, “Tumor angigoenesis: insights and inovations,” J. Oncolog. 2010, 132641 (2010).
  27. N. Mohtasham, S. Babakoohi, J. Salehinejad, L. Montaser-Kouhsari, M. T. Shakeri, S. Shojaee, N. S. Sistani, and A. Firooz, “Mast cell density and angiogenesis in oral dysplastic epithelium and low- and high-grade oral squamous cell carcinoma,” Acta Odontol. Scand. 68(5), 300–304 (2010). [CrossRef] [PubMed]
  28. E. Z. Michailidou, A. K. Markopoulos, and D. Z. Antoniades, “Mast cells and angiogenesis in oral malignant and premalignant lesions,” Open Dent. J. 2(1), 126–132 (2008). [CrossRef] [PubMed]
  29. M. Raica, A. M. Cimpean, and D. Ribatti, “Angiogenesis in pre-malignant conditions,” Eur. J. Cancer 45(11), 1924–1934 (2009). [CrossRef]

Cited By

 Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 3 Fig. 2
 
Fig. 4
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited