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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1296–1306

Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system

Steve Bégin, Bryan Burgoyne, Vincent Mercier, Alain Villeneuve, Réal Vallée, and Daniel Côté  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1296-1306 (2011)
http://dx.doi.org/10.1364/BOE.2.001296


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Abstract

We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700–2950 cm−1) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: January 6, 2011
Revised Manuscript: March 19, 2011
Manuscript Accepted: March 22, 2011
Published: April 22, 2011

Citation
Steve Bégin, Bryan Burgoyne, Vincent Mercier, Alain Villeneuve, Réal Vallée, and Daniel Côté, "Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system," Biomed. Opt. Express 2, 1296-1306 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1296


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