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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1307–1318

Position-referenced microscopy for live cell culture monitoring

July A. Galeano Z., Patrick Sandoz, Emilie Gaiffe, Sophie Launay, Laurent Robert, Maxime Jacquot, Fabienne Hirchaud, Jean-Luc Prétet, and Christiane Mougin  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1307-1318 (2011)
http://dx.doi.org/10.1364/BOE.2.001307


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Abstract

Position-referenced microscopy (PRM) is based on smart sample holders that integrate a position reference pattern (PRP) in their depth, allowing the determination of the lateral coordinates with respect to the sample-holder itself. Regions of interest can thus be retrieved easily after culture dish transfers from a cell incubator to the microscope stage. Images recorded at different instants in time are superimposed in a common coordinate system with subpixel accuracy. This paper presents such smart Petri culture dishes and their use for live cell culture monitoring. The impact of the PRP on the light budget is discussed and performances are demonstrated. First results on the application of PRM to the observation of apoptotic body internalization are reported.

© 2011 OSA

OCIS Codes
(100.0100) Image processing : Image processing
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Cell Studies

History
Original Manuscript: January 4, 2011
Revised Manuscript: April 13, 2011
Manuscript Accepted: April 14, 2011
Published: April 25, 2011

Citation
July A. Galeano Z., Patrick Sandoz, Emilie Gaiffe, Sophie Launay, Laurent Robert, Maxime Jacquot, Fabienne Hirchaud, Jean-Luc Prétet, and Christiane Mougin, "Position-referenced microscopy for live cell culture monitoring," Biomed. Opt. Express 2, 1307-1318 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1307


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