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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1340–1350

In vivo fluorescence lifetime optical projection tomography

James McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1340-1350 (2011)
http://dx.doi.org/10.1364/BOE.2.001340


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Abstract

We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.

© 2011 OSA

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Microscopy

History
Original Manuscript: March 4, 2011
Revised Manuscript: April 21, 2011
Manuscript Accepted: April 21, 2011
Published: April 26, 2011

Virtual Issues
July 22, 2011 Spotlight on Optics

Citation
James McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French, "In vivo fluorescence lifetime optical projection tomography," Biomed. Opt. Express 2, 1340-1350 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1340


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