OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1366–1376

Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy

Christian P. Pfeffer, Bjorn R. Olsen, Feruz Ganikhanov, and François Légaré  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1366-1376 (2011)
http://dx.doi.org/10.1364/BOE.2.001366


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Abstract

We describe experimental results on label free imaging of striated skeletal muscle using second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy. The complementarity of the SHG and CARS data makes it possible to clearly identify the main sarcomere sub-structures such as actin, myosin, acto-myosin, and the intact T-tubular system as it emanates from the sarcolemma. Owing to sub-micron spatial resolution and the high sensitivity of the CARS microscopy technique we were able to resolve individual myofibrils. In addition, key organelles such as mitochondria, cell nuclei and their structural constituents were observed revealing the entire structure of the muscle functional units. There is a noticeable difference in the CARS response of the muscle structure within actin, myosin and t-tubule areas with respect to laser polarization. We attribute this to a preferential alignment of the probed molecular bonds along certain directions. The combined CARS and SHG microscopy approach yields more extensive and complementary information and has a potential to become an indispensable method for live skeletal muscle characterization.

© 2011 OSA

OCIS Codes
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 25, 2011
Revised Manuscript: April 11, 2011
Manuscript Accepted: April 18, 2011
Published: April 27, 2011

Citation
Christian P. Pfeffer, Bjorn R. Olsen, Feruz Ganikhanov, and François Légaré, "Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy," Biomed. Opt. Express 2, 1366-1376 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1366


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