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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 6 — Jun. 1, 2011
  • pp: 1403–1411

Mouse phenotyping with near-infrared fluorescence lymphatic imaging

Sunkuk Kwon and Eva M. Sevick-Muraca  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 6, pp. 1403-1411 (2011)

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We demonstrate the ability to non-invasively and quantitatively image lymphatic architecture and contractile function using dynamic near-infrared (NIR) fluorescence imaging with injection of indocyanine green in normal and transgenic mice. Unlike normal mice, which showed well defined lymphatic drainage patterns and orthograde propagation of contraction waves, we observed tortuous and mispatterned lymphatic vessels and persistent retrograde lymph flow in mice with deficiency in Prox1, a transcription factor essential for lymphatic vascular development. NIR fluorescence imaging provides a method for quantifying lymphatic function for future studies designed to discern differences in lymphatic function in murine models of human lymphatic disease.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Functional Imaging

Original Manuscript: March 14, 2011
Revised Manuscript: April 20, 2011
Manuscript Accepted: April 29, 2011
Published: May 2, 2011

Virtual Issues
In vivo Microcirculation Imaging (2011) Biomedical Optics Express

Sunkuk Kwon and Eva M. Sevick-Muraca, "Mouse phenotyping with near-infrared fluorescence lymphatic imaging," Biomed. Opt. Express 2, 1403-1411 (2011)

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