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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 6 — Jun. 1, 2011
  • pp: 1504–1513

In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography

Dae Yu Kim, Jeff Fingler, John S. Werner, Daniel M. Schwartz, Scott E. Fraser, and Robert J. Zawadzki  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 6, pp. 1504-1513 (2011)
http://dx.doi.org/10.1364/BOE.2.001504


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Abstract

We present in vivo volumetric images of human retinal micro-circulation using Fourier-domain optical coherence tomography (Fd-OCT) with the phase-variance based motion contrast method. Currently fundus fluorescein angiography (FA) is the standard technique in clinical settings for visualizing blood circulation of the retina. High contrast imaging of retinal vasculature is achieved by injection of a fluorescein dye into the systemic circulation. We previously reported phase-variance optical coherence tomography (pvOCT) as an alternative and non-invasive technique to image human retinal capillaries. In contrast to FA, pvOCT allows not only noninvasive visualization of a two-dimensional retinal perfusion map but also volumetric morphology of retinal microvasculature with high sensitivity. In this paper we report high-speed acquisition at 125 kHz A-scans with pvOCT to reduce motion artifacts and increase the scanning area when compared with previous reports. Two scanning schemes with different sampling densities and scanning areas are evaluated to find optimal parameters for high acquisition speed in vivo imaging. In order to evaluate this technique, we compare pvOCT capillary imaging at 3x3 mm2 and 1.5x1.5 mm2 with fundus FA for a normal human subject. Additionally, a volumetric view of retinal capillaries and a stitched image acquired with ten 3x3 mm2 pvOCT sub-volumes are presented. Visualization of retinal vasculature with pvOCT has potential for diagnosis of retinal vascular diseases.

© 2011 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0110) Medical optics and biotechnology : Imaging systems
(170.4470) Medical optics and biotechnology : Ophthalmology

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: March 14, 2011
Revised Manuscript: May 9, 2011
Manuscript Accepted: April 27, 2011
Published: May 11, 2011

Citation
Dae Yu Kim, Jeff Fingler, John S. Werner, Daniel M. Schwartz, Scott E. Fraser, and Robert J. Zawadzki, "In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography," Biomed. Opt. Express 2, 1504-1513 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-6-1504


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