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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 6 — Jun. 1, 2011
  • pp: 1564–1575

Integrated holographic system for all-optical manipulation of developing embryos

Maria Leilani Torres-Mapa, Maciej Antkowiak, Hana Cizmarova, David E. K. Ferrier, Kishan Dholakia, and Frank J. Gunn-Moore  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 6, pp. 1564-1575 (2011)

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We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.

© 2011 OSA

OCIS Codes
(020.4180) Atomic and molecular physics : Multiphoton processes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.1420) Medical optics and biotechnology : Biology
(140.3538) Lasers and laser optics : Lasers, pulsed

ToC Category:
Developmental Biology

Original Manuscript: January 21, 2011
Revised Manuscript: April 17, 2011
Manuscript Accepted: May 13, 2011
Published: May 16, 2011

Maria Leilani Torres-Mapa, Maciej Antkowiak, Hana Cizmarova, David E. K. Ferrier, Kishan Dholakia, and Frank J. Gunn-Moore, "Integrated holographic system for all-optical manipulation of developing embryos," Biomed. Opt. Express 2, 1564-1575 (2011)

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