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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 6 — Jun. 1, 2011
  • pp: 1576–1583

In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature

Thomas E. Matthews, Jesse W. Wilson, Simone Degan, Mary Jane Simpson, Jane Y. Jin, Jennifer Y. Zhang, and Warren S. Warren  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 6, pp. 1576-1583 (2011)

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We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(320.7150) Ultrafast optics : Ultrafast spectroscopy

ToC Category:
Molecular Imaging and Probe Development

Original Manuscript: May 5, 2011
Revised Manuscript: May 13, 2011
Manuscript Accepted: May 13, 2011
Published: May 16, 2011

Thomas E. Matthews, Jesse W. Wilson, Simone Degan, Mary Jane Simpson, Jane Y. Jin, Jennifer Y. Zhang, and Warren S. Warren, "In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature," Biomed. Opt. Express 2, 1576-1583 (2011)

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