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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 7 — Jul. 1, 2011
  • pp: 1803–1814

Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level

Matti Kinnunen, Antti Kauppila, Artashes Karmenyan, and Risto Myllylä  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 7, pp. 1803-1814 (2011)
http://dx.doi.org/10.1364/BOE.2.001803


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Abstract

We demonstrate the use of a double-beam optical tweezers system to stabilize red blood cell (RBC) orientation in the optical tweezers during measurements of elastic light scattering from the trapped cells in an angle range of 5-30 degrees. Another laser (He-Ne) was used to illuminate the cell and elastic light scattering distribution from the single cell was measured with a goniometer and a photomultiplier tube. Moreover, CCD camera images of RBCs with and without laser illumination are presented as complementary information. Light scattering from a RBC was measured in different fixed orientations. Light scattering from cells was also measured when the length of the cell was changed in two different orientations. Light scattering measurements from spherical and crenate RBCs are described and the results are compared with other cell orientations. Analysis shows that the measured elastic light scattering distributions reveal changes in the RBC’s orientation and shape. The effect of stretching on the changes in scattering is larger in the case of face-on incidence of He-Ne laser light than in rim-on incidence. The scattering patterns from RBCs in different orientations as well as from a spherical RBC were compared with numerical results found in literature. Good correlation was found.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5850) Scattering : Scattering, particles
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Cell Studies

History
Original Manuscript: May 2, 2011
Revised Manuscript: May 26, 2011
Manuscript Accepted: May 31, 2011
Published: June 1, 2011

Citation
Matti Kinnunen, Antti Kauppila, Artashes Karmenyan, and Risto Myllylä, "Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level," Biomed. Opt. Express 2, 1803-1814 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-7-1803


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