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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 7 — Jul. 1, 2011
  • pp: 1931–1945

High-speed camera with real time processing for frequency domain imaging

Victor Shia, David Watt, and Gregory W. Faris  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 7, pp. 1931-1945 (2011)
http://dx.doi.org/10.1364/BOE.2.001931


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Abstract

We describe a high-speed camera system for frequency domain imaging suitable for applications such as in vivo diffuse optical imaging and fluorescence lifetime imaging. 14-bit images are acquired at 2 gigapixels per second and analyzed with real-time pipeline processing using field programmable gate arrays (FPGAs). Performance of the camera system has been tested both for RF-modulated laser imaging in combination with a gain-modulated image intensifier and a simpler system based upon an LED light source. System amplitude and phase noise are measured and compared against theoretical expressions in the shot noise limit presented for different frequency domain configurations. We show the camera itself is capable of shot noise limited performance for amplitude and phase in as little as 3 ms, and when used in combination with the intensifier the noise levels are nearly shot noise limited. The best phase noise in a single pixel is 0.04 degrees for a 1 s integration time.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5280) Medical optics and biotechnology : Photon migration
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Image Processing

History
Original Manuscript: May 2, 2011
Revised Manuscript: June 13, 2011
Manuscript Accepted: June 13, 2011
Published: June 15, 2011

Citation
Victor Shia, David Watt, and Gregory W. Faris, "High-speed camera with real time processing for frequency domain imaging," Biomed. Opt. Express 2, 1931-1945 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-7-1931


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References

  1. B. Chance, M. Cope, E. Gratton, N. Ramanujam, and B. Tromberg, “Phase measurement of light absorption and scatter in human tissue,” Rev. Sci. Instrum. 69(10), 3457–3481 (1998). [CrossRef]
  2. E. M. Sevick, B. Chance, J. Leigh, S. Nioka, and M. Maris, “Quantitation of time- and frequency-resolved optical spectra for the determination of tissue oxygenation,” Anal. Biochem. 195(2), 330–351 (1991). [CrossRef] [PubMed]
  3. S. J. Madsen, E. R. Anderson, R. C. Haskell, and B. J. Tromberg, “Portable, high-bandwidth frequency-domain photon migration instrument for tissue spectroscopy,” Opt. Lett. 19(23), 1934–1936 (1994). [CrossRef] [PubMed]
  4. H. Jiang, K. D. Paulsen, U. L. Osterberg, B. W. Pogue, and M. S. Patterson, “Simultaneous reconstruction of optical absorption and scattering maps in turbid media from near-infrared frequency-domain data,” Opt. Lett. 20(20), 2128–2130 (1995). [CrossRef] [PubMed]
  5. M. Gerken and G. W. Faris, “High-precision frequency-domain measurements of the optical properties of turbid media,” Opt. Lett. 24(14), 930–932 (1999). [CrossRef] [PubMed]
  6. V. Toronov, E. D’Amico, D. Hueber, E. Gratton, B. Barbieri, and A. Webb, “Optimization of the signal-to-noise ratio of frequency-domain instrumentation for near-infrared spectro-imaging of the human brain,” Opt. Express 11(21), 2717–2729 (2003). [CrossRef] [PubMed]
  7. A. B. Thompson and E. M. Sevick-Muraca, “Near-infrared fluorescence contrast-enhanced imaging with intensified charge-coupled device homodyne detection: measurement precision and accuracy,” J. Biomed. Opt. 8(1), 111–120 (2003). [CrossRef] [PubMed]
  8. S. V. Patwardhan and J. P. Culver, “Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier,” J. Biomed. Opt. 13(1), 011009 (2008). [CrossRef] [PubMed]
  9. T. French, J. Maier, and E. Gratton, “Frequency domain imaging of thick tissues using a CCD,” Proc. SPIE 1640, 254–261 (1992). [CrossRef]
  10. J. R. Lakowicz and K. W. Berndt, “Lifetime-selective fluorescence imaging using an rf phase-sensitive camera,” Rev. Sci. Instrum. 62(7), 1727–1734 (1991). [CrossRef]
  11. R. A. Colyer, C. Lee, and E. Gratton, “A novel fluorescence lifetime imaging system that optimizes photon efficiency,” Microsc. Res. Tech. 71(3), 201–213 (2008). [CrossRef] [PubMed]
  12. K. Zhang and J. U. Kang, “Real-time intraoperative 4D full-range FD-OCT based on the dual graphics processing units architecture for microsurgery guidance,” Biomed. Opt. Express 2(4), 764–770 (2011). [CrossRef] [PubMed]
  13. A. E. Desjardins, B. J. Vakoc, M. J. Suter, S. H. Yun, G. J. Tearney, and B. E. Bouma, “Real-time FPGA processing for high-speed optical frequency domain imaging,” IEEE Trans. Med. Imaging 28(9), 1468–1472 (2009). [CrossRef] [PubMed]
  14. J. R. Janesick, “Scientific Charge-Coupled Devices,” (SPIE Press, Bellingham, Washington, 2001), pp. 101–105.
  15. D. F. Walls, “Squeezed states of light,” Nature 306(5939), 141–146 (1983). [CrossRef]
  16. X. Gu, K. Ren, and A. H. Hielscher, “Frequency-domain sensitivity analysis for small imaging domains using the equation of radiative transfer,” Appl. Opt. 46(10), 1624–1632 (2007). [CrossRef] [PubMed]
  17. T. Tu, Y. Chen, J. Zhang, X. Intes, and B. Chance, “Analysis on performance and optimization of frequency-domain near-infrared instruments,” J. Biomed. Opt. 7(4), 643–649 (2002). [CrossRef] [PubMed]

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