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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2110–2116

Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy

Christoph Pohling, Tiago Buckup, Axel Pagenstecher, and Marcus Motzkus  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 8, pp. 2110-2116 (2011)
http://dx.doi.org/10.1364/BOE.2.002110


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Abstract

The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey and white matter as well as layers of granule and Purkinje cells. Additional quantitative information was obtained by using a decomposition algorithm. The results perfectly agree with HE stained references slides prepared separately making multiplex CARS an ideal approach for chemoselective imaging.

© 2011 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Microscopy

History
Original Manuscript: May 11, 2011
Revised Manuscript: June 27, 2011
Manuscript Accepted: June 27, 2011
Published: June 30, 2011

Citation
Christoph Pohling, Tiago Buckup, Axel Pagenstecher, and Marcus Motzkus, "Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy," Biomed. Opt. Express 2, 2110-2116 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-8-2110


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