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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2117–2125

Fiber-optic system for monitoring fast photoactivation dynamics of optical highlighter fluorescent proteins

Zhiguo Pei, Lingsong Qin, Zhihong Zhang, Shaoqun Zeng, and Zhen-Li Huang  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2117-2125 (2011)

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Characterizing the photoactivation performance of optical highlighter fluorescent proteins is crucial to the realization of photoactivation localization microscopy. In contrast to those fluorescence-based approaches that require complex data processing and calibration procedures, here we report a simple and quantitative alternative, which relies on the measurement of small absorption spectra changes over time with a fiber-optic system. Using Dronpa as a representative highlighter protein, we have investigated the capacity of this system in monitoring the fast photoactivation process.

© 2011 OSA

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(300.1030) Spectroscopy : Absorption

ToC Category:
Optical Biophysics

Original Manuscript: May 9, 2011
Revised Manuscript: June 28, 2011
Manuscript Accepted: July 1, 2011
Published: July 1, 2011

Zhiguo Pei, Lingsong Qin, Zhihong Zhang, Shaoqun Zeng, and Zhen-Li Huang, "Fiber-optic system for monitoring fast photoactivation dynamics of optical highlighter fluorescent proteins," Biomed. Opt. Express 2, 2117-2125 (2011)

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