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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2231–2242

Optimization of pupil design for point-scanning and line-scanning confocal microscopy

Yogesh G. Patel, Milind Rajadhyaksha, and Charles A. DiMarzio  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2231-2242 (2011)

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Both point-scanning and line-scanning confocal microscopes provide resolution and optical sectioning to observe nuclear and cellular detail in human tissues, and are being translated for clinical applications. While traditional point-scanning is truly confocal and offers the best possible optical sectioning and resolution, line-scanning is partially confocal but may offer a relatively simpler and lower-cost alternative for more widespread dissemination into clinical settings. The loss of sectioning and loss of contrast due to scattering in tissue is more rapid and more severe with a line-scan than with a point-scan. However, the sectioning and contrast may be recovered with the use of a divided-pupil. Thus, as part of our efforts to translate confocal microscopy for detection of skin cancer, and to determine the best possible approach for clinical applications, we are now developing a quantitative understanding of imaging performance for a set of scanning and pupil conditions. We report a Fourier-analysis-based computational model of confocal microscopy for six configurations. The six configurations are point-scanning and line-scanning, with full-pupil, half-pupil and divided-pupils. The performance, in terms of on-axis irradiance (signal), resolution and sectioning capabilities, is quantified and compared among these six configurations.

© 2011 OSA

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:

Original Manuscript: May 2, 2011
Revised Manuscript: July 6, 2011
Manuscript Accepted: July 7, 2011
Published: July 8, 2011

Yogesh G. Patel, Milind Rajadhyaksha, and Charles A. DiMarzio, "Optimization of pupil design for point-scanning and line-scanning confocal microscopy," Biomed. Opt. Express 2, 2231-2242 (2011)

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