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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2255–2264

Cell assay using a two-photon-excited europium chelate

Xudong Xiao, Jeanne P. Haushalter, Kenneth T. Kotz, and Gregory W. Faris  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2255-2264 (2011)

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We report application of two-photon excitation of europium chelates to immunolabeling of epidermal growth factor receptor (EGFR) cell surface proteins on A431 cancer cells. The europium chelates are excited with two photons of infrared light and emit in the visible. Europium chelates are conjugated to antibodies for EGFR. A431 (human epidermoid carcinoma) cells are labeled with this conjugate and imaged using a multiphoton microscope. To minimize signal loss due to the relatively long-lived Eu3+ emission, the multiphoton microscope is used with scanning laser two-photon excitation and non-scanning detection with a CCD. The chelate labels show very little photobleaching (less than 1% during continuous illumination in the microscope for 20 minutes) and low levels of autofluorescence (less than 1% of the signal from labeled cells). The detection limit of the europium label in the cell assay is better than 100 zeptomoles.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.7220) Nonlinear optics : Upconversion
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 2, 2011
Revised Manuscript: June 13, 2011
Manuscript Accepted: July 11, 2011
Published: July 13, 2011

Xudong Xiao, Jeanne P. Haushalter, Kenneth T. Kotz, and Gregory W. Faris, "Cell assay using a two-photon-excited europium chelate," Biomed. Opt. Express 2, 2255-2264 (2011)

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