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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2279–2287

In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy

Hideyuki Watanabe, Uma Maheswari Rajagopalan, Yu Nakamichi, Kei M. Igarashi, Violeta Dimitrova Madjarova, Hirofumi Kadono, and Manabu Tanifuji  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2279-2287 (2011)

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Here, we report in vivo 3-D visualization of the layered organization of a rat olfactory bulb (OB) by a swept source optical coherence tomography (SS-OCT). The SS-OCT operates at a wavelength of 1334 nm with respective theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm in air and hence it is possible to get a 3D structural map of OB in vivo at the micron level resolution with millimeter-scale imaging depth. Up until now, with methods such as MRI, confocal microscopy, OB depth structure in vivo had not been clearly visualized as these do not satisfy the criterion of simultaneously providing micron-scale spatial resolution and imaging up to a few millimeter in depth. In order to confirm the OB’s layered organization revealed by SS-OCT, we introduced the technique of electrocoagulation to make landmarks across the layered structure. To our knowledge this is such a first study that combines electrocoagulation and OCT in vivo of rat OB. Our results confirmed the layered organization of OB, and moreover the layers were clearly identified by electrocoagulation landmarks both in the OCT structural and anatomical slice images. We expect such a combined study is beneficial for both OCT and neuroscience fields.

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.5380) Medical optics and biotechnology : Physiology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

Original Manuscript: June 6, 2011
Revised Manuscript: July 13, 2011
Manuscript Accepted: July 13, 2011
Published: July 15, 2011

Hideyuki Watanabe, Uma Maheswari Rajagopalan, Yu Nakamichi, Kei M. Igarashi, Violeta Dimitrova Madjarova, Hirofumi Kadono, and Manabu Tanifuji, "In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy," Biomed. Opt. Express 2, 2279-2287 (2011)

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