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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2299–2306

How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte

Ahmed Alrifaiy and Kerstin Ramser  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2299-2306 (2011)

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We present a new concept of integrating a micropipette within a closed microfluidic system equipped with optical tweezers and a UV-Vis spectrometer. A single red blood cell (RBC) was optically trapped and steered in three dimensions towards a micropipette that was integrated in the microfluidic system. Different oxygenation states of the RBC, triggered by altering the oxygen content in the microchannels through a pump system, were optically monitored by a UV-Vis spectrometer. The built setup is aimed to act as a multifunctional system where the biochemical content and the electrophysiological reaction of a single cell can be monitored simultaneously. The system can be used for other applications like single cell sorting, in vitro fertilization or electrophysiological experiments with precise environmental control of the gas-, and chemical content.

© 2011 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(220.4000) Optical design and fabrication : Microstructure fabrication
(280.2490) Remote sensing and sensors : Flow diagnostics
(300.1030) Spectroscopy : Absorption
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:

Original Manuscript: May 23, 2011
Revised Manuscript: June 30, 2011
Manuscript Accepted: July 14, 2011
Published: July 20, 2011

Ahmed Alrifaiy and Kerstin Ramser, "How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte," Biomed. Opt. Express 2, 2299-2306 (2011)

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