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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2364–2371

Two-color STED microscopy in living cells

Patrina A. Pellett, Xiaoli Sun, Travis J. Gould, James E. Rothman, Ming-Qun Xu, Ivan R. Corrêa, Jr., and Joerg Bewersdorf  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 8, pp. 2364-2371 (2011)
http://dx.doi.org/10.1364/BOE.2.002364


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Abstract

Diffraction-unlimited resolution provided by Stimulated Emission Depletion (STED) microscopy allows for imaging cellular processes in living cells that are not visible by conventional microscopy. However, it has so far not been possible to study dynamic nanoscale interactions because multicolor live cell STED microscopy has yet to be demonstrated and suitable labeling technologies and protocols are lacking. Here we report the first realization of two-color STED imaging in living cells. Using improved SNAPf and CLIPf technologies to label epidermal growth factor (EGF) and EGF receptor (EGFR), we report resolutions of 78 nm and 82 nm for 22 sequential two-color scans in living cells.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: June 15, 2011
Revised Manuscript: July 21, 2011
Manuscript Accepted: July 21, 2011
Published: July 22, 2011

Citation
Patrina A. Pellett, Xiaoli Sun, Travis J. Gould, James E. Rothman, Ming-Qun Xu, Ivan R. Corrêa, and Joerg Bewersdorf, "Two-color STED microscopy in living cells," Biomed. Opt. Express 2, 2364-2371 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-8-2364


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