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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2484–2492

Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis

Jeon Woong Kang, Niyom Lue, Chae-Ryon Kong, Ishan Barman, Narahara C. Dingari, Stephen J. Goldfless, Jacquin C. Niles, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 9, pp. 2484-2492 (2011)
http://dx.doi.org/10.1364/BOE.2.002484


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Abstract

We have developed a novel multimodal microscopy system that incorporates confocal Raman, confocal reflectance, and quantitative phase microscopy (QPM) into a single imaging entity. Confocal Raman microscopy provides detailed chemical information from the sample, while confocal reflectance and quantitative phase microscopy show detailed morphology. Combining these intrinsic contrast imaging modalities makes it possible to obtain quantitative morphological and chemical information without exogenous staining. For validation and characterization, we have used this multi-modal system to investigate healthy and diseased blood samples. We first show that the thickness of a healthy red blood cell (RBC) shows good correlation with its hemoglobin distribution. Further, in malaria infected RBCs, we successfully image the distribution of hemozoin (malaria pigment) inside the cell. Our observations lead us to propose morphological screening by QPM and subsequent chemical imaging by Raman for investigating blood disorders. This new approach allows monitoring cell development and cell-drug interactions with minimal perturbation of the biological system of interest.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 26, 2011
Revised Manuscript: June 30, 2011
Manuscript Accepted: July 29, 2011
Published: August 1, 2011

Citation
Jeon Woong Kang, Niyom Lue, Chae-Ryon Kong, Ishan Barman, Narahara C. Dingari, Stephen J. Goldfless, Jacquin C. Niles, Ramachandra R. Dasari, and Michael S. Feld, "Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis," Biomed. Opt. Express 2, 2484-2492 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-9-2484


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