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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2504–2516

Changes to lipid droplet configuration in mCMV-infected fibroblasts: live cell imaging with simultaneous CARS and two-photon fluorescence microscopy

Christine S. Y. Wong, Iain Robinson, Michael A. Ochsenkühn, Jochen Arlt, William J. Hossack, and Jason Crain  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 9, pp. 2504-2516 (2011)

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We have performed multimodal imaging of live fibroblast cells infected by murine cytomegalovirus (mCMV). The infection process was monitored by imaging the two-photon fluorescence signal from a GFP-expressing strain of mCMV, whilst changes to lipid droplet configuration were observed by CARS imaging. This allowed us to identify three visually distinct stages of infection. Quantitative analysis of lipid droplet number and size distributions were obtained from live cells, which showed significant perturbations across the different stages of infection. The CARS and two-photon images were acquired simultaneously and the experimental design allowed incorporation of an environmental control chamber to maintain cell viability. Photodamage to the live cell population was also assessed.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing

ToC Category:
Cell Studies

Original Manuscript: May 9, 2011
Revised Manuscript: June 5, 2011
Manuscript Accepted: July 8, 2011
Published: August 2, 2011

Christine S. Y. Wong, Iain Robinson, Michael A. Ochsenkühn, Jochen Arlt, William J. Hossack, and Jason Crain, "Changes to lipid droplet configuration in mCMV-infected fibroblasts: live cell imaging with simultaneous CARS and two-photon fluorescence microscopy," Biomed. Opt. Express 2, 2504-2516 (2011)

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