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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2517–2531

Effects of incomplete decay in fluorescence lifetime estimation

Regina Won Kay Leung, Shu-Chi Allison Yeh, and Qiyin Fang  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 9, pp. 2517-2531 (2011)

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Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results.

© 2011 OSA

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: May 2, 2011
Revised Manuscript: July 21, 2011
Manuscript Accepted: July 31, 2011
Published: August 2, 2011

Regina Won Kay Leung, Shu-Chi Allison Yeh, and Qiyin Fang, "Effects of incomplete decay in fluorescence lifetime estimation," Biomed. Opt. Express 2, 2517-2531 (2011)

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