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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2540–2550

In vivo three-dimensional spectroscopic photoacoustic imaging for monitoring nanoparticle delivery

Seungsoo Kim, Yun-Sheng Chen, Geoffrey P. Luke, and Stanislav Y. Emelianov  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 9, pp. 2540-2550 (2011)

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In vivo monitoring of nanoparticle delivery is essential to better understand cellular and molecular interactions of nanoparticles with tissue and to better plan nanoparticle-mediated therapies. We developed a three-dimensional ultrasound and photoacoustic (PA) imaging system and a spectroscopic PA imaging algorithm to identify and quantify the presence of nanoparticles and other tissue constituents. Using the developed system and approach, three-dimensional in vivo imaging of a mouse with tumor was performed before and after intravenous injection of gold nanorods. The developed spectroscopic PA imaging algorithm estimated distribution of nanoparticle as well as oxygen saturation of blood. Moreover, silver staining of excised tumor tissue confirmed nanoparticle deposition, and showed good correlation with spectroscopic PA images. The results of our study suggest that three-dimensional ultrasound-guided spectroscopic PA imaging can monitor nanoparticle delivery in vivo.

© 2011 OSA

OCIS Codes
(110.7170) Imaging systems : Ultrasound
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: May 2, 2011
Revised Manuscript: July 29, 2011
Manuscript Accepted: August 3, 2011
Published: August 5, 2011

Seungsoo Kim, Yun-Sheng Chen, Geoffrey P. Luke, and Stanislav Y. Emelianov, "In vivo three-dimensional spectroscopic photoacoustic imaging for monitoring nanoparticle delivery," Biomed. Opt. Express 2, 2540-2550 (2011)

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