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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2590–2599

Holographic UV laser microsurgery

Aroshan K Jayasinghe, Jason Rohner, and M Shane Hutson  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 9, pp. 2590-2599 (2011)
http://dx.doi.org/10.1364/BOE.2.002590


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Abstract

We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate the ability of this single-pulse multi-point system to perform two experiments that are very difficult for conventional microsurgery—isolating single cells in vivo and measuring fast retractions from large incisions.

© 2011 OSA

OCIS Codes
(170.1020) Medical optics and biotechnology : Ablation of tissue
(090.1995) Holography : Digital holography
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Optical Therapies and Photomodificaton

History
Original Manuscript: July 1, 2011
Revised Manuscript: August 9, 2011
Manuscript Accepted: August 9, 2011
Published: August 15, 2011

Citation
Aroshan K Jayasinghe, Jason Rohner, and M Shane Hutson, "Holographic UV laser microsurgery," Biomed. Opt. Express 2, 2590-2599 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-9-2590


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