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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2665–2678

Evaluation of a spectrally resolved scattering microscope

Michael Schmitz, Thomas Rothe, and Alwin Kienle  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 9, pp. 2665-2678 (2011)

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A scattering microscope was developed to investigate single cells and biological microstructures by light scattering measurements. The spectrally resolved part of the setup and its validation are shown in detail. The analysis of light scattered by homogenous polystyrene spheres allows the determination of their diameters using Mie theory. The diameters of 150 single polystyrene spheres were determined by the spectrally resolved scattering microscope. In comparison, the same polystyrene suspension stock was investigated by a collimated transmission setup. Mean diameters and standard deviations of the size distribution were evaluated by both methods with a statistical error of less than 1nm. The systematic errors of both devices are in agreement within the measurement accuracy.

© 2011 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(290.1350) Scattering : Backscattering
(290.2200) Scattering : Extinction
(290.4020) Scattering : Mie theory
(290.5850) Scattering : Scattering, particles
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:

Original Manuscript: July 6, 2011
Revised Manuscript: August 2, 2011
Manuscript Accepted: August 16, 2011
Published: August 23, 2011

Michael Schmitz, Thomas Rothe, and Alwin Kienle, "Evaluation of a spectrally resolved scattering microscope," Biomed. Opt. Express 2, 2665-2678 (2011)

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