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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 1 — Jan. 1, 2012
  • pp: 153–159

Measurement of the traction force of biological cells by digital holography

Xiao Yu, Michael Cross, Changgeng Liu, David C. Clark, Donald T. Haynie, and Myung K. Kim  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 1, pp. 153-159 (2012)
http://dx.doi.org/10.1364/BOE.3.000153


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Abstract

The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular traction force have been measured from phase profiles in a direct and straightforward manner. DH-QPM is shown to provide highly efficient and versatile means for quantitatively analyzing cellular motility.

© 2011 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(090.1995) Holography : Digital holography

ToC Category:
Cell Studies

History
Original Manuscript: November 9, 2011
Revised Manuscript: December 2, 2011
Manuscript Accepted: December 14, 2011
Published: December 16, 2011

Citation
Xiao Yu, Michael Cross, Changgeng Liu, David C. Clark, Donald T. Haynie, and Myung K. Kim, "Measurement of the traction force of biological cells by digital holography," Biomed. Opt. Express 3, 153-159 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-1-153


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References

  1. A. K. Harris, P. Wild, and D. Stopak, “Silicone rubber substrata: a new wrinkle in the study of cell locomotion,” Science208(4440), 177–179 (1980). [CrossRef] [PubMed]
  2. J. Lee, M. Leonard, T. Oliver, A. Ishihara, and K. Jacobson, “Traction forces generated by locomoting keratocytes,” J. Cell Biol.127(6), 1957–1964 (1994). [CrossRef] [PubMed]
  3. T. Oliver, K. Jacobson, and M. Dembo, “Traction forces in locomoting cells,” Cell Motil. Cytoskeleton31(3), 225–240 (1995). [CrossRef] [PubMed]
  4. M. Dembo and Y. L. Wang, “Stresses at the cell-to-substrate interface during locomotion of fibroblasts,” Biophys. J.76(4), 2307–2316 (1999). [CrossRef] [PubMed]
  5. C. M. Lo, H. B. Wang, M. Dembo, and Y. L. Wang, “Cell movement is guided by the rigidity of the substrate,” Biophys. J.79(1), 144–152 (2000). [CrossRef] [PubMed]
  6. T. Oliver, J. Lee, and K. Jacobson, “Forces exerted by locomoting cells,” Semin. Cell Biol.5(3), 139–147 (1994). [CrossRef] [PubMed]
  7. S. Usami, S. L. Wung, B. A. Skierczynski, R. Skalak, and S. Chien, “Locomotion forces generated by a polymorphonuclear leukocyte,” Biophys. J.63(6), 1663–1666 (1992). [CrossRef] [PubMed]
  8. E. K. Dimitriadis, F. Horkay, J. Maresca, B. Kachar, and R. S. Chadwick, “Determination of elastic moduli of thin layers of soft material using the atomic force microscope,” Biophys. J.82(5), 2798–2810 (2002). [CrossRef] [PubMed]
  9. D. C. Lin, B. Yurke, and N. A. Langrana, “Use of rigid spherical inclusions in Young’s moduli determination: application to DNA-crosslinked gels,” J. Biomech. Eng.127(4), 571–579 (2005). [CrossRef] [PubMed]
  10. M. K. Kim, “Principles and techniques of digital holographic microscopy,” SPIE Rev.1, 1–50 (2010).
  11. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett.24(5), 291–293 (1999). [CrossRef] [PubMed]
  12. C. J. Mann, L. F. Yu, C. M. Lo, and M. K. Kim, “High-resolution quantitative phase-contrast microscopy by digital holography,” Opt. Express13(22), 8693–8698 (2005). [CrossRef] [PubMed]
  13. A. K. Harris, University of North Carolina at Chapel Hill, (personal communication).
  14. C. Liu, Y. S. Bae, W. Z. Yang, and D. Y. Kim, “All-in-one multifunctional optical microscope with a single holographic measurement,” Opt. Eng.47(8), 087001 (2008). [CrossRef]

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