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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 1 — Jan. 1, 2012
  • pp: 206–214

Improving signal-to-noise ratio of structured light microscopy based on photon reassignment

Vijay Raj Singh, Heejin Choi, Elijah Y. S. Yew, Dipanjan Bhattacharya, Luo Yuan, Colin J. R. Sheppard, Jagath C. Rajapakse, George Barbastathis, and Peter T. C. So  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 1, pp. 206-214 (2012)
http://dx.doi.org/10.1364/BOE.3.000206


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Abstract

In this paper, we report a method for 3D visualization of a biological specimen utilizing a structured light wide-field microscopic imaging system. This method improves on existing structured light imaging modalities by reassigning fluorescence photons generated from off-focal plane excitation, improving in-focus signal strength. Utilizing a maximum likelihood approach, we identify the most likely fluorophore distribution in 3D that will produce the observed image stacks under structured and uniform illumination using an iterative maximization algorithm. Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods.

© 2011 OSA

OCIS Codes
(100.3020) Image processing : Image reconstruction-restoration
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 16, 2011
Revised Manuscript: December 11, 2011
Manuscript Accepted: December 11, 2011
Published: December 22, 2011

Virtual Issues
Advances in Optics for Biotechnology, Medicine, and Surgery (2011) Biomedical Optics Express

Citation
Vijay Raj Singh, Heejin Choi, Elijah Y. S. Yew, Dipanjan Bhattacharya, Luo Yuan, Colin J. R. Sheppard, Jagath C. Rajapakse, George Barbastathis, and Peter T. C. So, "Improving signal-to-noise ratio of structured light microscopy based on photon reassignment," Biomed. Opt. Express 3, 206-214 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-1-206


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References

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