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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 1 — Jan. 1, 2012
  • pp: 55–63

Optical coherence tomography-based freeze-drying microscopy

Mircea Mujat, Kristyn Greco, Kristin L. Galbally-Kinney, Daniel X. Hammer, R. Daniel Ferguson, Nicusor Iftimia, Phillip Mulhall, Puneet Sharma, Michael J. Pikal, and William J. Kessler  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 1, pp. 55-63 (2012)

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A new type of freeze-drying microscope based upon time-domain optical coherence tomography is presented here (OCT-FDM). The microscope allows for real-time, in situ 3D imaging of pharmaceutical formulations in vials relevant for manufacturing processes with a lateral resolution of <7 μm and an axial resolution of <5 μm. Correlation of volumetric structural imaging with product temperature measured during the freeze-drying cycle allowed investigation of structural changes in the product and determination of the temperature at which the freeze-dried cake collapses. This critical temperature is the most important parameter in designing freeze-drying processes of pharmaceutical products.

© 2011 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4500) Imaging systems : Optical coherence tomography
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Optical Coherence Tomography

Original Manuscript: November 9, 2011
Revised Manuscript: December 1, 2011
Manuscript Accepted: December 2, 2011
Published: December 7, 2011

Mircea Mujat, Kristyn Greco, Kristin L. Galbally-Kinney, Daniel X. Hammer, R. Daniel Ferguson, Nicusor Iftimia, Phillip Mulhall, Puneet Sharma, Michael J. Pikal, and William J. Kessler, "Optical coherence tomography-based freeze-drying microscopy," Biomed. Opt. Express 3, 55-63 (2012)

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