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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 10 — Oct. 1, 2012
  • pp: 2395–2404

Retrieving the optical parameters of biological tissues using diffuse reflectance spectroscopy and Fourier series expansions. I. theory and application

Aarón A. Muñoz Morales and Sergio Vázquez y Montiel  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 10, pp. 2395-2404 (2012)

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The determination of optical parameters of biological tissues is essential for the application of optical techniques in the diagnosis and treatment of diseases. Diffuse Reflection Spectroscopy is a widely used technique to analyze the optical characteristics of biological tissues. In this paper we show that by using diffuse reflectance spectra and a new mathematical model we can retrieve the optical parameters by applying an adjustment of the data with nonlinear least squares. In our model we represent the spectra using a Fourier series expansion finding mathematical relations between the polynomial coefficients and the optical parameters. In this first paper we use spectra generated by the Monte Carlo Multilayered Technique to simulate the propagation of photons in turbid media. Using these spectra we determine the behavior of Fourier series coefficients when varying the optical parameters of the medium under study. With this procedure we find mathematical relations between Fourier series coefficients and optical parameters. Finally, the results show that our method can retrieve the optical parameters of biological tissues with accuracy that is adequate for medical applications.

© 2012 OSA

OCIS Codes
(170.7050) Medical optics and biotechnology : Turbid media
(290.3200) Scattering : Inverse scattering
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optics of Tissue and Turbid Media

Original Manuscript: May 24, 2012
Revised Manuscript: June 23, 2012
Manuscript Accepted: June 30, 2012
Published: September 5, 2012

Aarón A. Muñoz Morales and Sergio Vázquez y Montiel, "Retrieving the optical parameters of biological tissues using diffuse reflectance spectroscopy and Fourier series expansions. I. theory and application," Biomed. Opt. Express 3, 2395-2404 (2012)

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