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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 10 — Oct. 1, 2012
  • pp: 2587–2599

In vivo absorption spectroscopy for absolute measurement

Hiromitsu Furukawa and Takashi Fukuda  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 10, pp. 2587-2599 (2012)

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In in vivo spectroscopy, there are differences between individual subjects in parameters such as tissue scattering and sample concentration. We propose a method that can provide the absolute value of a particular substance concentration, independent of these individual differences. Thus, it is not necessary to use the typical statistical calibration curve, which assumes an average level of scattering and an averaged concentration over individual subjects. This method is expected to greatly reduce the difficulties encountered during in vivo measurements. As an example, for in vivo absorption spectroscopy, the method was applied to the reflectance measurement in retinal vessels to monitor their oxygen saturation levels. This method was then validated by applying it to the tissue phantom under a variety of absorbance values and scattering efficiencies.

© 2012 OSA

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.1030) Spectroscopy : Absorption

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: May 30, 2012
Revised Manuscript: August 17, 2012
Manuscript Accepted: September 14, 2012
Published: September 18, 2012

Hiromitsu Furukawa and Takashi Fukuda, "In vivo absorption spectroscopy for absolute measurement," Biomed. Opt. Express 3, 2587-2599 (2012)

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  1. M. Born and E. Wolf, “Beam propagation in an absorbing medium,” in Principles of Optics 7th Ed. (Cambridge University Press, Cambridge, 2002).
  2. J. H. Lambert, Photometria sive de mensura et gradibus luminus, colorum et umbrae (1760) [Published in German by E. Anding under the title Lambert’s Photometrie, (Verlag von Wilhelm Engelmann, Leipzig, 1892)].
  3. A. Beer, “Bestimmung der Absorption des rothen Lichts in farbigen Flüssigkeiten,” Annal. Phys. Chem.86, 78–88 (1852).
  4. J. M. Beach, K. J. Schwenzer, S. Srinivas, D. Kim, and J. S. Tiedeman, “Oximetry of retinal vessels by dual-wavelength imaging: calibration and influence of pigmentation,” J. Appl. Physiol.86(2), 748–758 (1999). [PubMed]
  5. M. H. Smith, K. R. Denninghoff, A. Lompado, and L. W. Hillman, “Effect of multiple light paths on retinal vessel oximetry,” Appl. Opt.39(7), 1183–1193 (2000). [CrossRef] [PubMed]
  6. A. Kienle, M. S. Patterson, N. Dögnitz, R. Bays, G. Wagnieres, and H. van den Bergh, “Noninvasive determination of the optical properties of two-layered turbid media,” Appl. Opt.37(4), 779–791 (1998). [CrossRef] [PubMed]
  7. H. M. Heise, “Applications of near-infrared spectroscopy in medical sciences,” Near-Infrared Spectroscopy, H. W. Siesler, Y. Ozeki, S. Kawata, and H. M. Heise ed. (Wiley VCH Verlag GmbH, Weinheim, 2002).
  8. A. J. Cohen and R. A. Laing, “Multiple scattering analysis of retinal blood oximetry,” IEEE Trans. Biomed. Eng.BME-23(5), 391–400 (1976). [CrossRef] [PubMed]
  9. S. Prahl, “Optical absorption of hemoglobin.” http://omlc.ogi.edu/spectra/hemoglobin .
  10. P. Kubelka and F. Munk, ““Ein Beitrag zur Optik der Farbanstriche,” Zeits. f,” Tech. Phys.12, 593–601 (1931).
  11. S. Traustason, A. S. Jensen, H. S. Arvidsson, I. C. Munch, L. Søndergaard, and M. Larsen, “Retinal oxygen saturation in patients with systemic hypoxemia,” Invest. Ophthalmol. Vis. Sci.52(8), 5064–5067 (2011). [CrossRef] [PubMed]
  12. Medical electrical equipment Part 2: Particular requirements for the safety of diagnostic and therapeutic laser equipment, IEC 60101–2-22 and IEC 60825–1. Japanese Industrial Standards, JIS C 6802–2005.
  13. J. V. B. Soares, J. J. G. Leandro, R. M. Cesar, H. F. Jelinek, and M. J. Cree, “Retinal vessel segmentation using the 2-D Gabor wavelet and supervised classification,” IEEE Trans. Med. Imaging25(9), 1214–1222 (2006). [CrossRef] [PubMed]
  14. F. C. Mokken, F. J. M. van der Waart, C. P. Henny, P. T. Goedhart, and A. W. Gelb, “Differences in peripheral arterial and venous hemorheologic parameters,” Ann. Hematol.73(3), 135–137 (1996). [CrossRef] [PubMed]
  15. J. M. Steinke and A. P. Shepherd, “Comparison of Mie theory and the light scattering of red blood cells,” Appl. Opt.27(19), 4027–4033 (1988). [CrossRef] [PubMed]
  16. M. S. Patterson, B. C. Wilson, and D. R. Wyman, “The propagation of optical radiation in tissue I. Models of radiation transport and their application,” Lasers Med. Sci.6(2), 155–168 (1991). [CrossRef]
  17. D. Link, C. Strohmaier, B. U. Seifert, T. Riemer, H. A. Reitsamer, J. Haueisen, and W. Vilser, “Novel non-contact retina camera for the rat and its application to dynamic retinal vessel analysis,” Biomed. Opt. Express2(11), 3094–3108 (2011). [CrossRef] [PubMed]
  18. J. W. Severinghaus and Y. Honda, “History of blood gas analysis. VII. Pulse oximetry,” J. Clin. Monit.3(2), 135–138 (1987). [CrossRef] [PubMed]

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