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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2752–2760

Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy

Jesse W. Wilson, Simone Degan, Warren S. Warren, and Martin C. Fischer  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 11, pp. 2752-2760 (2012)

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Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.

© 2012 OSA

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Ophthalmology Applications

Original Manuscript: August 23, 2012
Revised Manuscript: September 28, 2012
Manuscript Accepted: October 1, 2012
Published: October 3, 2012

Jesse W. Wilson, Simone Degan, Warren S. Warren, and Martin C. Fischer, "Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy," Biomed. Opt. Express 3, 2752-2760 (2012)

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Supplementary Material

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