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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2860–2865

Imaging morphodynamics of human blood cells in vivo with video-rate third harmonic generation microscopy

Chien-Kuo Chen and Tzu-Ming Liu  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 11, pp. 2860-2865 (2012)

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With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every now and then, round and granule rich blood cells with strong THG contrast appear in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts.

© 2012 OSA

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics

ToC Category:

Original Manuscript: June 12, 2012
Revised Manuscript: August 30, 2012
Manuscript Accepted: September 13, 2012
Published: October 17, 2012

Virtual Issues
BIOMED 2012 (2012) Biomedical Optics Express

Chien-Kuo Chen and Tzu-Ming Liu, "Imaging morphodynamics of human blood cells in vivo with video-rate third harmonic generation microscopy," Biomed. Opt. Express 3, 2860-2865 (2012)

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