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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2866–2880

Dynamic quantitative phase imaging for biological objects using a pixelated phase mask

Katherine Creath and Goldie Goldstein  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 11, pp. 2866-2880 (2012)
http://dx.doi.org/10.1364/BOE.3.002866


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Abstract

This paper describes research in developing a dynamic quantitative phase imaging microscope providing instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns derived using the polarization properties of light to track dynamic motions and morphological changes. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Two different processing routines are presented to remove background surface shape to enable quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented.

© 2012 OSA

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy
(110.3175) Imaging systems : Interferometric imaging

ToC Category:
Microscopy

History
Original Manuscript: June 26, 2012
Revised Manuscript: August 25, 2012
Manuscript Accepted: September 11, 2012
Published: October 17, 2012

Virtual Issues
BIOMED 2012 (2012) Biomedical Optics Express

Citation
Katherine Creath and Goldie Goldstein, "Dynamic quantitative phase imaging for biological objects using a pixelated phase mask," Biomed. Opt. Express 3, 2866-2880 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-11-2866


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