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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2881–2895

In vivo photothermal optical coherence tomography of gold nanorod contrast agents

J. M. Tucker-Schwartz, T. A. Meyer, C. A. Patil, C. L. Duvall, and M. C. Skala  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 11, pp. 2881-2895 (2012)

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Photothermal optical coherence tomography (PT-OCT) is a potentially powerful tool for molecular imaging. Here, we characterize PT-OCT imaging of gold nanorod (GNR) contrast agents in phantoms, and we apply these techniques for in vivo GNR imaging. The PT-OCT signal was compared to the bio-heat equation in phantoms, and in vivo PT-OCT images were acquired from subcutaneous 400 pM GNR Matrigel injections into mice. Experiments revealed that PT-OCT signals varied as predicted by the bio-heat equation, with significant PT-OCT signal increases at 7.5 pM GNR compared to a scattering control (p < 0.01) while imaging in common path configuration. In vivo PT-OCT images demonstrated an appreciable increase in signal in the presence of GNRs compared to controls. Additionally, in vivo PT-OCT GNR signals were spatially distinct from blood vessels imaged with Doppler OCT. We anticipate that the demonstrated in vivo PT-OCT sensitivity to GNR contrast agents is sufficient to image molecular expression in vivo. Therefore, this work demonstrates the translation of PT-OCT to in vivo imaging and represents the next step towards its use as an in vivo molecular imaging tool.

© 2012 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(350.5340) Other areas of optics : Photothermal effects
(160.4236) Materials : Nanomaterials

ToC Category:
Optical Coherence Tomography

Original Manuscript: September 5, 2012
Revised Manuscript: October 12, 2012
Manuscript Accepted: October 15, 2012
Published: October 17, 2012

J. M. Tucker-Schwartz, T. A. Meyer, C. A. Patil, C. L. Duvall, and M. C. Skala, "In vivo photothermal optical coherence tomography of gold nanorod contrast agents," Biomed. Opt. Express 3, 2881-2895 (2012)

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