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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2964–2975

Standoff detection of biological agents using laser induced fluorescence—a comparison of 294 nm and 355 nm excitation wavelengths

Øystein Farsund, Gunnar Rustad, and Gunnar Skogan  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 11, pp. 2964-2975 (2012)

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Standoff detection measuring the fluorescence spectra of seven different biological agents excited by 294 nm as well as 355 nm wavelength laser pulses has been undertaken. The biological warfare agent simulants were released in a semi-closed aerosol chamber at 210 m standoff distance and excited by light at either of the two wavelengths using the same instrument. Significant differences in several of the agents’ fluorescence response were seen at the two wavelengths. The anthrax simulants’ fluorescence responses were almost an order of magnitude stronger at the shorter wavelength excitation. However, most importantly, the fluorescence spectra were significantly more dissimilar at 294 nm than at 355 nm excitation with ~7 nm spectral resolution. This indicates that classification of the substances should be possible with a lower error rate for standoff detection using 294 nm rather than 355 nm excitation wavelength, or even better, utilizing both.

© 2012 OSA

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(280.1100) Remote sensing and sensors : Aerosol detection
(280.3640) Remote sensing and sensors : Lidar
(300.2530) Spectroscopy : Fluorescence, laser-induced
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Biodefense and Bioterrorism

Original Manuscript: August 30, 2012
Revised Manuscript: October 15, 2012
Manuscript Accepted: October 16, 2012
Published: October 24, 2012

Øystein Farsund, Gunnar Rustad, and Gunnar Skogan, "Standoff detection of biological agents using laser induced fluorescence—a comparison of 294 nm and 355 nm excitation wavelengths," Biomed. Opt. Express 3, 2964-2975 (2012)

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