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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2976–2986

Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice

Zhongwei Zhi, Xin Yin, Suzan Dziennis, Tomasz Wietecha, Kelly L. Hudkins, Charles E. Alpers, and Ruikang K Wang  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 11, pp. 2976-2986 (2012)
http://dx.doi.org/10.1364/BOE.3.002976


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Abstract

We present a novel application of optical microangiography (OMAG) imaging technique for visualization of depth-resolved vascular network within retina and choroid as well as measurement of total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a line scan rate of 140 kHz was developed to image the posterior segment of eyes in mice. By applying an OMAG algorithm to extract the moving blood flow signals out of the background tissue, we are able to provide true capillary level imaging of the retinal and choroidal vasculature. The microvascular patterns within different retinal layers are presented. An en face Doppler OCT approach [Srinivasan et al., Opt Express 18, 2477 (2010)] was adopted for retinal blood flow measurement. The flow is calculated by integrating the axial blood flow velocity over the vessel area measured in an en face plane without knowing the blood vessel angle. Total retinal blood flow can be measured from both retinal arteries and veins. The results indicate that OMAG has the potential for qualitative and quantitative evaluation of the microcirculation in posterior eye compartments in mouse models of retinopathy and neovascularization.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: September 6, 2012
Revised Manuscript: October 12, 2012
Manuscript Accepted: October 18, 2012
Published: October 24, 2012

Citation
Zhongwei Zhi, Xin Yin, Suzan Dziennis, Tomasz Wietecha, Kelly L. Hudkins, Charles E. Alpers, and Ruikang K Wang, "Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice," Biomed. Opt. Express 3, 2976-2986 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-11-2976


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