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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 3022–3032

4D shear stress maps of the developing heart using Doppler optical coherence tomography

Lindsy M. Peterson, Michael W. Jenkins, Shi Gu, Lee Barwick, Michiko Watanabe, and Andrew M. Rollins  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 11, pp. 3022-3032 (2012)
http://dx.doi.org/10.1364/BOE.3.003022


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Abstract

Accurate imaging and measurement of hemodynamic forces is vital for investigating how physical forces acting on the embryonic heart are transduced and influence developmental pathways. Of particular importance is blood flow-induced shear stress, which influences gene expression by endothelial cells and potentially leads to congenital heart defects through abnormal heart looping, septation, and valvulogenesis. However no imaging tool has been available to measure shear stress on the endocardium volumetrically and dynamically. Using 4D structural and Doppler OCT imaging, we are able to accurately measure the blood flow in the heart tube in vivo and to map endocardial shear stress throughout the heart cycle under physiological conditions for the first time. These measurements of the shear stress patterns will enable precise titration of experimental perturbations and accurate correlation of shear with the expression of molecules critical to heart development.

© 2012 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: August 22, 2012
Revised Manuscript: October 11, 2012
Manuscript Accepted: October 15, 2012
Published: October 31, 2012

Citation
Lindsy M. Peterson, Michael W. Jenkins, Shi Gu, Lee Barwick, Michiko Watanabe, and Andrew M. Rollins, "4D shear stress maps of the developing heart using Doppler optical coherence tomography," Biomed. Opt. Express 3, 3022-3032 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-11-3022


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