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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 273–281

Mitochondrial redox studies of oxidative stress in kidneys from diabetic mice

Sepideh Maleki, Reyhaneh Sepehr, Kevin Staniszewski, Nader Sheibani, Christine M. Sorenson, and Mahsa Ranji  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 2, pp. 273-281 (2012)

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Chronic hyperglycemia during diabetes leads to increased production of reactive oxygen species (ROS) and increased oxidative stress (OS). Here we investigated whether changes in the metabolic state can be used as a marker of OS progression in kidneys. We examined redox states of kidneys from diabetic mice, Akita/+ and Akita/+;TSP1–/– mice (Akita mice lacking thrombospondin-1, TSP1) with increasing duration of diabetes. OS as measured by mitochondrial redox ratio (NADH/FAD) was detectable shortly after the onset of diabetes and further increased with the duration of diabetes. Thus, cryo fluorescence redox imaging was used as a quantitative marker of OS progression in kidneys from diabetic mice and demonstrated that alterations in the oxidative state of kidneys occur during the early stages of diabetes.

© 2012 OSA

OCIS Codes
(000.1430) General : Biology and medicine
(100.2960) Image processing : Image analysis
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(260.2510) Physical optics : Fluorescence

ToC Category:
Functional Imaging

Original Manuscript: November 23, 2011
Revised Manuscript: December 16, 2011
Manuscript Accepted: January 1, 2012
Published: January 10, 2012

Sepideh Maleki, Reyhaneh Sepehr, Kevin Staniszewski, Nader Sheibani, Christine M. Sorenson, and Mahsa Ranji, "Mitochondrial redox studies of oxidative stress in kidneys from diabetic mice," Biomed. Opt. Express 3, 273-281 (2012)

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