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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 313–326

Enhanced robustness digital holographic microscopy for demanding environment of space biology

M. Fatih Toy, Stéphane Richard, Jonas Kühn, Alfredo Franco-Obregón, Marcel Egli, and Christian Depeursinge  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 2, pp. 313-326 (2012)
http://dx.doi.org/10.1364/BOE.3.000313


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Abstract

We describe an optimized digital holographic microscopy system (DHM) suitable for high-resolution visualization of living cells under conditions of altered macroscopic mechanical forces such as those that arise from changes in gravitational force. Experiments were performed on both a ground-based microgravity simulation platform known as the random positioning machine (RPM) as well as during a parabolic flight campaign (PFC). Under these conditions the DHM system proved to be robust and reliable. In addition, the stability of the system during disturbances in gravitational force was further enhanced by implementing post-processing algorithms that best exploit the intrinsic advantages of DHM for hologram autofocusing and subsequent image registration. Preliminary results obtained in the form of series of phase images point towards sensible changes of cytoarchitecture under states of altered gravity.

© 2012 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: October 25, 2011
Revised Manuscript: January 6, 2012
Manuscript Accepted: January 12, 2012
Published: January 13, 2012

Citation
M. Fatih Toy, Stéphane Richard, Jonas Kühn, Alfredo Franco-Obregón, Marcel Egli, and Christian Depeursinge, "Enhanced robustness digital holographic microscopy for demanding environment of space biology," Biomed. Opt. Express 3, 313-326 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-2-313


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